Rabbit anti-Human CWC22 Polyclonal Antibody | anti-CWC22 antibody

CWC22, NT (CWC22, KIAA1604, NCM, Pre-mRNA-splicing factor CWC22 homolog, Nucampholin homolog, fSAPb) (PE)

Cat. Num. AAA6290154

• Gene Names: CWC22; NCM; fSAPb; EIF4GL
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CWC22; Polyclonal Antibody; NT (CWC22; KIAA1604; NCM; Pre-mRNA-splicing factor CWC22 homolog; Nucampholin homolog; fSAPb) (PE); fSAPb; anti-CWC22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CWC22 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CWC22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human CWC22.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CWC22 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CWC22 antibody detected the CWC22 protein (arrow).)

Immunohistochemistry (IHC)

(CWC22 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CWC22 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CWC22 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CWC22 antibody

Antibodies; Splicing Factors

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Olsen, J.V., et al. Cell 127(3):635-648(2006). Lehner, B., et al. Genome Res. 14(7):1315-1323(2004). Jurica, M.S., et al. RNA 8(4):426-439(2002). Kelly, W.G., et al. Genetics 146(1):227-238(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 55749769
• NCBI GeneID: 57703
• NCBI Accession #: NP_065994.1
• NCBI GenBank Nucleotide #: NM_020943.2
• UniProt Accession #: Q9HCG8; Q05DC2; Q4G135; Q52LF0; Q6PEX2; Q7Z6I0; Q9H5L3; Q9H6Q6
• Molecular Weight: 105,466 Da
• NCBI Official Full Name: pre-mRNA-splicing factor CWC22 homolog
• NCBI Official Synonym Full Names: CWC22 spliceosome associated protein homolog
• NCBI Official Symbol: CWC22
• NCBI Official Synonym Symbols: NCM; fSAPb; EIF4GL
• NCBI Protein Information: pre-mRNA-splicing factor CWC22 homolog
• UniProt Protein Name: Pre-mRNA-splicing factor CWC22 homolog
• Protein Family: Pre-mRNA-splicing factor
• UniProt Gene Name: CWC22
• UniProt Synonym Gene Names: KIAA1604; NCM

Uniprot Description

Required for pre-mRNA splicing and for exon-junction complex (EJC) assembly. Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay.