Rabbit CXCL9 Polyclonal Antibody | anti-CXCL9 antibody

RABBIT ANTI HUMAN CXCL9

Cat. Num. AAA223923

• Gene Names: CXCL9; CMK; MIG; Humig; SCYB9; crg-10
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: CXCL9; Polyclonal Antibody; RABBIT ANTI HUMAN CXCL9; anti-CXCL9 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 125

• Applicable Applications for anti-CXCL9 antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;; Functional Assays: Minimum Dilution: 5.0; Maximum Dilution: 7.0ug/ml;; Immunohistology - Paraffin; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
• Immunogen: Recombinant human CXCL9; Buffer Solution
• Antiserum Preparation: Antiserum to human CXCL9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (MBS223923) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Testing Data #2

(Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (MBS223923) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-CXCL9 antibody

Rabbit anti Human CXCL9 polyclonal antinbody recognizes human CXCL9, otherwise known as MIG (monokine induced by Interferon-gamma), a member of the CXC family of chemokine receptors, secreted by interferon stimulated monocytes, macrophages (Muller et al. 2010) and endothelial cells (Loos et al. 2006) CXCL9 signals through binding to CD183 (CXCR3). CXCL9 acts as a Th1 (type 1 helper T) cell chemoattractant, and plays a role in the growth, activation and movement of cells associated with immune and inflammatory responses, and in tumour growth inhibition and angiogenesis (Whiting et al. 2004). Studies have shown that CXCL9 is active against E Coli, S.aureus, L.monocytogenes (Cole et al. 2001) and S.pyogenes (Egesten et al. 2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4505187
• NCBI GeneID: 4283
• NCBI Accession #: NP_002407.1
• NCBI GenBank Nucleotide #: NM_002416.2
• UniProt Accession #: Q07325; Q503B4
• Molecular Weight: 14,019 Da
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Official Synonym Symbols: CMK; MIG; Humig; SCYB9; crg-10
• NCBI Protein Information: C-X-C motif chemokine 9; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma; small-inducible cytokine B9
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL9
• UniProt Synonym Gene Names: CMK; MIG; SCYB9; HuMIG; MIG
• UniProt Entry Name: CXCL9_HUMAN

NCBI Description

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; defense response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; immune response; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; chemotaxis; inflammatory response; signal transduction; defense response to virus; positive regulation of myoblast differentiation; regulation of cell proliferation