Human CXCL9 ELISA Kit | CXCL9 elisa kit

Human CXCL9 PicoKine ELISA Kit

Cat. Num. AAA176008

• Gene Names: CXCL9; CMK; MIG; Humig; SCYB9; crg-10
• Reactivity: Human
• Synonyms: CXCL9; Human CXCL9 PicoKine ELISA Kit; C-X-C motif chemokine 9; chemokine (C-X-C motif) ligand 9; Chemokine (C-X-C motif) ligand 9; CMK; Crg 10; CXCL9_HUMAN; Gamma interferon induced monokine; Gamma-interferon-induced monokine; HuMIG; MIG; Monokine induced by gamma interferon; Monokine induced by interferon gamma; Monokine induced by interferon-gamma; SCYB9; Small inducible cytokine B9; Small inducible cytokine subfamily B member 9; Small-inducible cytokine B9; CXCL9 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CXCL9
• Sequence Length: 125

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 2pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CXCL9 PicoKine ELISA Kit standard curve )

Related Product Information for CXCL9 elisa kit

Principle of the assay: human CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL9 has been precoated onto 96-well plates. Standards (E.coli,T23-T125) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL9 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotacticfunctions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinanthuman CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 585487
• NCBI GeneID: 4283
• UniProt Accession #: Q07325; Q503B4
• Molecular Weight: 14,019 Da
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Official Synonym Symbols: CMK; MIG; Humig; SCYB9; crg-10
• NCBI Protein Information: C-X-C motif chemokine 9; small-inducible cytokine B9; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL9
• UniProt Synonym Gene Names: CMK; MIG; SCYB9; HuMIG; MIG
• UniProt Entry Name: CXCL9_HUMAN

NCBI Description

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; defense response; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; positive regulation of myoblast differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; defense response to virus