Rabbit CXADR Polyclonal Antibody | anti-CXADR antibody

CXADR Polyclonal Antibody

Cat. Num. AAA3005725

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CXADR; Polyclonal Antibody; CXADR Polyclonal Antibody; Coxsackievirus and adenovirus receptor; CAR; Hcar; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR; anti-CXADR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CXADR polyclonal antibody detects endogenous levels of CXADR protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 252

• Applicable Applications for anti-CXADR antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 48-93 of Human CXADR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CXADR polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-CXADR antibody

The coxsackie and adenovirus receptor (CAR) mediates viral infection by the binding of various adenoviruses through specific protein interactions. There is a high affinity between the viral knob domain and the extracellular amino- terminal domain (designated D1) of CAR. The D1 domain alone is sufficient for knob binding in transfected cells. Determining the specific interactions between CAR and adenoviruses is imperative in order to further develop gene therapy using viral hosts. CAR is expressed in many human and murine cell types. However, cells that express CAR at low levels are not efficiently infected by adenoviruses. Possible methods of avoiding this problem in certain cell types are by either supplementing CAR or modifying the Ad knob to bind to other receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 333470703
• NCBI GeneID: 1525
• NCBI Accession #: NP_001193992.1
• NCBI GenBank Nucleotide #: NP_001193992.1
• UniProt Accession #: P78310
• Molecular Weight: ~ 46kDa
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 2
• NCBI Official Synonym Full Names: CXADR Ig-like cell adhesion molecule
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: CXADR-like membrane protein
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR
• UniProt Entry Name: CXAR_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; basolateral plasma membrane; apicolateral plasma membrane; acrosome; extracellular region; intercellular junction; lipid raft; nucleoplasm; adherens junction; growth cone; plasma membrane; neuromuscular junction; cell junction; filopodium

Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: mitochondrion organization and biogenesis; neutrophil chemotaxis; intercellular junction assembly and maintenance; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; germ cell migration; homotypic cell-cell adhesion; blood coagulation; defense response to virus; leukocyte migration