Rabbit anti-Human, Pig CAR Polyclonal Antibody | anti-CAR antibody

CAR polyclonal antibody

Cat. Num. AAA3015844

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Reactivity: Human, Pig
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: CAR; Polyclonal Antibody; CAR polyclonal antibody; Coxsackievirus and adenovirus receptor; hCAR; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR; CXADR; anti-CAR antibody

• Host: Rabbit
• Reactivity: Human, Pig
• Clonality: Polyclonal
• Specificity: CAR polyclonal antibody detects endogenous levels of CAR protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-CAR antibody: Western Blot (WB)
• Application Notes: WB: 1:1000~1:2000
• Immunogen: Synthetic peptide, corresponding to Human CAR.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CAR polyclonal antibody at 1:1000 dilution Lane1:EC9706 whole cell lysate(40ug) Lane2:The Lung tissue lysate of Pig(40ug) Lane3:The Heart tissue lysate of Pig(40ug) Lane4:LOVO whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(20ug))

Related Product Information for anti-CAR antibody

The coxsackie virus and adenovirus receptor (CXADR, CAR) is a highly conserved, single-transmembrane glycoprotein and the primary receptor to mediate cellular attachment and infection of coxsackie B viruses and most adenoviruses. The CAR protein contains a pair of Ig-like domains within the amino-terminal extracellular domain and a carboxyl-terminal PDZ motif. Research studies indicate that CAR is a tight junction protein that associates with the ZO-1 scaffold protein and promotes both cell adhesion and restriction of solute and ion movement between cells. Endogenous CAR is targeted to the basolateral plasma membrane by a tyrosine-based basolateral sorting signal and clathrin adaptors AP-1A and AP-1B. CAR binds junctional adhesion molecule L (JAML) on epithelial cells and neutrophils where it activates PI3K and downstream MAPK kinases to stimulate epithelial gammadelta T cell proliferation and increase production of TNFalpha and keratinocyte growth factor. As a result, the CAR protein plays a potentially critical role in adenoviral gene therapy, immunity, wound repair, inflammation, and cancer therapy. Additional studies demonstrate that CAR is essential in regulating squamous carcinoma cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 333470703
• NCBI GeneID: 1525
• NCBI Accession #: NP_001193992.1
• NCBI GenBank Nucleotide #: NM_001207063.1
• UniProt Accession #: P78310; O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8
• Molecular Weight: 40,030 Da
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 2
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor; HCVADR; CVB3-binding protein; coxsackievirus B-adenovirus receptor; 46 kD coxsackievirus and adenovirus receptor (CAR) protein
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: Carabin
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR
• UniProt Entry Name: CXAR_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; basolateral plasma membrane; apicolateral plasma membrane; acrosome; extracellular region; intercellular junction; lipid raft; nucleoplasm; adherens junction; growth cone; plasma membrane; neuromuscular junction; cell junction; filopodium

Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: mitochondrion organization and biogenesis; neutrophil chemotaxis; intercellular junction assembly and maintenance; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; germ cell migration; homotypic cell-cell adhesion; blood coagulation; defense response to virus; leukocyte migration