CXADR/CAR Active Protein | CXADR active protein

Human CellExp CXADR / CAR, human recombinant

Cat. Num. AAA844913

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: CXADR/CAR; Human CellExp CXADR / CAR; human recombinant; CXADR; CAR; CAR4/6; HCAR; CXADR active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 252

• Applicable Applications for CXADR active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 mug/mL and 100 muL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37 degree C.
• Results: Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 mug/mL and 100 muL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CXADR active protein

Background: Coxsackie virus and adenovirus receptor (CXADR) also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V- type (D1) and a C2- type (D2) Ig-like domain, a transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.

Product Categories/Family for CXADR active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 333470703
• NCBI GeneID: 1525
• NCBI Accession #: NP_001193992.1
• NCBI GenBank Nucleotide #: NM_001207063.1
• UniProt Accession #: P78310; O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8; B7WPI3; D3YHP0
• Molecular Weight: This protein is fused with a C-terminal 6×His tag, has a calculated MW of 24.9 kDa expressed. The predicted N-terminus is Leu 20. Protein migrates as 35 kDa in reduced SDS-PAGE due to glycosylation.
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 2
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: Coxsackievirus and adenovirus receptor
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR
• UniProt Entry Name: CXAR_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Receptor, misc.; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; tight junction; protein complex; integral to plasma membrane; apicolateral plasma membrane; basolateral plasma membrane; extracellular region; acrosome; intercellular junction; lipid raft; nucleoplasm; growth cone; adherens junction; plasma membrane; cell junction; neuromuscular junction; filopodium

Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; neutrophil chemotaxis; mitochondrion organization and biogenesis; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; homotypic cell-cell adhesion; germ cell migration; blood coagulation; leukocyte migration; defense response to virus