Western Blot (WB)
(CWC22 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CWC22 antibody detected the CWC22 protein (arrow).)
Rabbit anti-Human CWC22 Polyclonal Antibody | anti-CWC22 antibody
CWC22 Antibody (N-term)
Gene Names
CWC22; NCM; fSAPb; EIF4GL
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CWC22; Polyclonal Antibody; CWC22 Antibody (N-term); Pre-mRNA-splicing factor CWC22 homolog; Nucampholin homolog; fSAPb; KIAA1604; NCM; anti-CWC22 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CWC22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human CWC22.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
16-45
Sequence Length
908
Applicable Applications for anti-CWC22 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(CWC22 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CWC22 antibody detected the CWC22 protein (arrow).)
Western Blot (WB)
(CWC22 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CWC22 antibody detected the CWC22 protein (arrow).)
Immunohistochemistry (IHC)
(CWC22 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CWC22 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CWC22 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CWC22 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CWC22 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CWC22 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CWC22 antibody
May be involved in pre-mRNA splicing.
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Jurica, M.S., et al. RNA 8(4):426-439(2002)
Kelly, W.G., et al. Genetics 146(1):227-238(1997)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Jurica, M.S., et al. RNA 8(4):426-439(2002)
Kelly, W.G., et al. Genetics 146(1):227-238(1997)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105466
NCBI Official Full Name
pre-mRNA-splicing factor CWC22 homolog
NCBI Official Synonym Full Names
CWC22 spliceosome-associated protein
NCBI Official Symbol
CWC22
NCBI Official Synonym Symbols
NCM; fSAPb; EIF4GL
NCBI Protein Information
pre-mRNA-splicing factor CWC22 homolog
UniProt Protein Name
Pre-mRNA-splicing factor CWC22 homolog
Protein Family
UniProt Gene Name
CWC22
UniProt Synonym Gene Names
KIAA1604; NCM
UniProt Entry Name
CWC22_HUMAN
Uniprot Description
NCM: a component of the spliceosome C complex.
Protein type: RNA processing; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 2q31.3
Cellular Component: nucleoplasm; Golgi apparatus; spliceosome; cytoplasm; nuclear speck
Molecular Function: protein binding; RNA binding
Biological Process: nuclear mRNA splicing, via spliceosome; regulation of nuclear mRNA splicing, via spliceosome
Research Articles on CWC22
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Product Notes
The CWC22 cwc22 (Catalog #AAA9214492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 16-45. The CWC22 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CWC22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the CWC22 cwc22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CWC22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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