Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CWC22 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit anti-Mouse CWC22 Polyclonal Antibody | anti-CWC22 antibody
CWC22 Polyclonal Antibody
Gene Names
CWC22; NCM; fSAPb; EIF4GL
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
CWC22; Polyclonal Antibody; CWC22 Polyclonal Antibody; EIF4GL; fSAPb; NCM; anti-CWC22 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
LTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESK
Sequence Length
908
Applicable Applications for anti-CWC22 antibody
Western Blot (WB)
Application Notes
WB: 1:1000 - 1:2000
Immunogen
Recombinant protein of human CWC22
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Nucleus, Nucleus speckle
Positive Samples
Mouse liver, Mouse testis
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CWC22 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CWC22 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Product Categories/Family for anti-CWC22 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 105kDa
Observed: 110kDa
Observed: 110kDa
NCBI Official Full Name
pre-mRNA-splicing factor CWC22 homolog
NCBI Official Synonym Full Names
CWC22 spliceosome associated protein homolog
NCBI Official Symbol
CWC22
NCBI Official Synonym Symbols
NCM; fSAPb; EIF4GL
NCBI Protein Information
pre-mRNA-splicing factor CWC22 homolog
UniProt Protein Name
Pre-mRNA-splicing factor CWC22 homolog
Protein Family
UniProt Gene Name
CWC22
UniProt Synonym Gene Names
KIAA1604; NCM
Uniprot Description
Required for pre-mRNA splicing and for exon-junction complex (EJC) assembly. Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay.
Research Articles on CWC22
Similar Products
Product Notes
The CWC22 cwc22 (Catalog #AAA9133164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CWC22 Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CWC22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 - 1:2000. Researchers should empirically determine the suitability of the CWC22 cwc22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LTDELREHLK NTPKVIVAQK PDVEQNKSSP SSSSSASSSS ESDSSDSDSD SSDSSSESSS EESDSSSISS HSSASANDVR KKGHGKTRSK EVDKLIRNQQ TNDRKQKERR QEHGHQETRT ERERRSEKHR DQNSSGSNWR DPITKYTSDK DVPSERNNYS RVANDRDQEM HIDLENKHGD PKKKRGERRN SFSENEKHTH RIKDSENFRR KDRSKSKEMN RKHSGSRSDE DRYQNGAERR WEKSSRYSEQ SRESK. It is sometimes possible for the material contained within the vial of "CWC22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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