Western blot (WB)
(Western blot analysis of HIF-2 alpha onSiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.)
Rabbit HIF-2 alpha Monoclonal Antibody | anti-HIF2A antibody
HIF-2 alpha Antibody
Gene Names
EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Pro A affinity purified
Synonyms
HIF-2 alpha; Monoclonal Antibody; HIF-2 alpha Antibody; Basic helix loop helix PAS protein MOP2 antibody; Basic-helix-loop-helix-PAS protein MOP2 antibody; bHLHe73 antibody; Class E basic helix-loop-helix protein 73 antibody; ECYT4 antibody; Endothelial PAS domain containing protein 1 antibody; Endothelial pas domain protein 1 antibody; Endothelial PAS domain-containing protein 1 antibody; EPAS 1 antibody; EPAS-1 antibody; EPAS1 antibody; EPAS1_HUMAN antibody.; anti-HIF2A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
JB24-42
Purity/Purification
Pro A affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Sequence Length
870
Applicable Applications for anti-HIF2A antibody
Western Blot (WB), Immunohistochemsitry (IHC), Flow Cytometry (FC).
Application Notes
WB: 1:500-1:2,000; IHC: 1:50-1:200; FC: 1:50-1:100
Immunogen
Recombinant protein
Subcellular location
Nucleus.
Positive control
SiHa, rat lung tissue, human breast cancer tissue, human placenta tissue, mouse colon tissue, HUVEC.
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles.
Western blot (WB)
(Western blot analysis of HIF-2 alpha onSiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.)
Western blot (WB)
(Western blot analysis of HIF-2 alpha onSiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraffin embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraffin embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.)
Related Product Information for anti-HIF2A antibody
Hypoxia-inducible factors, including HIF-1Alpha, HIF-1Beta (also designated Arnt 1), EPAS-1 (also designated HIF-2Alpha) and HIF-3Alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96 kDa
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
NCBI Protein Information
endothelial PAS domain-containing protein 1
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Gene Name
EPAS1
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
UniProt Entry Name
EPAS1_HUMAN
NCBI Description
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
Research Articles on HIF2A
Similar Products
Product Notes
The HIF2A epas1 (Catalog #AAA8565763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF-2 alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HIF-2 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemsitry (IHC), Flow Cytometry (FC). WB: 1:500-1:2,000; IHC: 1:50-1:200; FC: 1:50-1:100. Researchers should empirically determine the suitability of the HIF2A epas1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF-2 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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