Rabbit CTSK Polyclonal Antibody | anti-CTSK antibody

CTSK Polyclonal Antibody

Cat. Num. AAA127614

• Gene Names: CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• Reactivity: Mouse, Rat, Duck
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CTSK; Polyclonal Antibody; CTSK Polyclonal Antibody; CTS02; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; anti-CTSK antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Duck
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 329

• Applicable Applications for anti-CTSK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CTSK
• Calculated Molecular Weight: 39kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of mouse liver, using CTSK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using CTSK antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using CTSK antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using CTSK antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-CTSK antibody

CTSK(cathepsin K), also named as CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts(PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot(PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. This antibody is specific to CTSK.

Product Categories/Family for anti-CTSK antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 1168793
• NCBI GeneID: 1513
• UniProt Accession #: P43235; Q6FHS6
• Molecular Weight: 329
• NCBI Official Full Name: Cathepsin K
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Official Synonym Symbols: CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• NCBI Protein Information: cathepsin K; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: CTSK
• UniProt Synonym Gene Names: CTSO; CTSO2
• UniProt Entry Name: CATK_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Uniprot Description

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.22.38

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; extracellular region

Molecular Function: collagen binding; protein binding; proteoglycan binding; cysteine-type endopeptidase activity; fibronectin binding; cysteine-type peptidase activity

Biological Process: extracellular matrix disassembly; collagen catabolic process; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption

Disease: Pycnodysostosis