Human cathepsin K ELISA Kit | CTSK elisa kit

Human Cathepsin K, Cath-K ELISA Kit

Cat. Num. AAA700377

• Gene Names: CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• Reactivity: Human
• Synonyms: cathepsin K; Human Cathepsin K; Cath-K ELISA Kit; cath-K ELISA Kit; RP11-363I22.4; CTS02; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; cathepsin O1; cathepsin O2; cathepsin X; CTSK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human cath-K. No significant cross-reactivity or interference between human cath-K and analogues was observed.
• Sequence Length: 329

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8 pg/ml -500 pg/ml
• Sensitivity: <1.95 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSK elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cath-K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cath-K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cath-K is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cath-K bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503151
• NCBI GeneID: 1513
• NCBI Accession #: NP_000387.1
• NCBI GenBank Nucleotide #: NM_000396.3
• UniProt Accession #: P43235; Q6FHS6
• Molecular Weight: 36,966 Da
• NCBI Official Full Name: cathepsin K preproprotein
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Official Synonym Symbols: CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• NCBI Protein Information: cathepsin K; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: CTSK
• UniProt Synonym Gene Names: CTSO; CTSO2
• UniProt Entry Name: CATK_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Uniprot Description

Function: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

Catalytic activity: Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.