Typical Testing Data/Standard Curve (for reference only)
Bovine Cathepsin K ELISA Kit | CTSK elisa kit
Bovine Cathepsin K ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Cath-K ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Cath-K antibody and an Cath-K-HRP conjugate. The assay sample and buffer are incubated together with Cath-K-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Cath-K concentration since Cath-K from samples and Cath-K-HRP conjugate compete for the anti-Cath-K antibody binding site. Since the number of sites is limited, as more sites are occupied by Cath-K from the sample, fewer sites are left to bind Cath-K-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cath-K concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]
Uniprot Description
CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.
Protein type: Protease; EC 3.4.22.38
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: extracellular space; extracellular region
Molecular Function: collagen binding; protein binding; proteoglycan binding; cysteine-type endopeptidase activity; fibronectin binding; cysteine-type peptidase activity
Biological Process: extracellular matrix disassembly; collagen catabolic process; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption
Disease: Pycnodysostosis
Research Articles on CTSK
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Product Notes
The Bovine CTSK ctsk (Catalog #AAA741765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741765 ELISA Kit recognizes Bovine CTSK. It is sometimes possible for the material contained within the vial of "Cathepsin K, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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