Rabbit CTCF Polyclonal Antibody | anti-CTCF antibody

CTCF Polyclonal Antibody

Cat. Num. AAA3007537

• Gene Names: CTCF; MRD21; FAP108; CFAP108
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CTCF; Polyclonal Antibody; CTCF Polyclonal Antibody; 11 zinc finger protein; 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC binding factor (zinc finger protein); CCCTC binding factor; CCCTC-binding factor; Ctcf; CTCFL paralog; Transcriptional repressor CTCF; anti-CTCF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CTCF polyclonal antibody detects endogenous levels of CTCF protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 727

• Applicable Applications for anti-CTCF antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:50-1:100; CHIP: 1:50-1:100
• Immunogen: Recombinant full length Human CTCF.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CTCF polyclonal antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody.)

Related Product Information for anti-CTCF antibody

CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2-class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development.

NCBI and Uniprot Product Information

• NCBI GI #: 1706179
• NCBI GeneID: 10664
• UniProt Accession #: P49711
• Molecular Weight: ~ 140kDa
• NCBI Official Full Name: Transcriptional repressor CTCF
• NCBI Official Synonym Full Names: CCCTC-binding factor
• NCBI Official Symbol: CTCF
• NCBI Official Synonym Symbols: MRD21; FAP108; CFAP108
• NCBI Protein Information: transcriptional repressor CTCF
• UniProt Protein Name: Transcriptional repressor CTCF
• Protein Family: Transcriptional repressor
• UniProt Gene Name: CTCF
• UniProt Entry Name: CTCF_HUMAN

NCBI Description

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.

Protein type: Tumor suppressor; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 16q21-q22.3

Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; chromatin insulator sequence binding; transcription factor activity; transcription corepressor activity

Biological Process: genetic imprinting; transcription, DNA-dependent; maintenance of DNA methylation; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 21