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Typical Testing Data/Standard Curve (for reference only)

Human Stratifin (SFN) ELISA Kit | SFN elisa kit

Human Stratifin (SFN) ELISA Kit

Gene Names
SFN; YWHAS
Reactivity
Human
Synonyms
Stratifin (SFN); Human Stratifin (SFN) ELISA Kit; SFN elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
248
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
3ng/ml - 900ng/ml
Sensitivity
1.53ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SFN elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Stratifin (also known as SFN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SFN antibody. SFN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SFN Antibody is added and binds to SFN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SFN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,336 Da
NCBI Official Full Name
Stratifin
NCBI Official Synonym Full Names
stratifin
NCBI Official Symbol
SFN
NCBI Official Synonym Symbols
YWHAS
NCBI Protein Information
14-3-3 protein sigma
UniProt Protein Name
14-3-3 protein sigma
Protein Family
UniProt Gene Name
SFN
UniProt Synonym Gene Names
HME1

NCBI Description

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.

Research Articles on SFN

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Product Notes

The Human SFN sfn (Catalog #AAA161662) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161662 ELISA Kit recognizes Human SFN. It is sometimes possible for the material contained within the vial of "Stratifin (SFN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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