Rabbit CTCF Polyclonal Antibody | anti-CTCF antibody

CTCF Polyclonal Antibody

Cat. Num. AAA127321

• Gene Names: CTCF; MRD21
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CTCF; Polyclonal Antibody; CTCF Polyclonal Antibody; anti-CTCF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 727

• Applicable Applications for anti-CTCF antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CTCF
• Calculated Molecular Weight: 83kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-CTCF antibody

CCCTC-binding factor (CTCF) and its paralog, the Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved transcription factors that regulate transcriptional activation and repression, insulator function, and imprinting control regions (ICRs) (1-4). Although they have divergent amino and carboxy termini, both proteins contain 11 conserved zinc finger domains that work in combination to bind the same DNA elements (1). CTCF is ubiquitously expressed and contributes to transcriptional regulation of cell-growth regulated genes, including c-myc, p19/ARF, p16/INK4A, BRCA1, p53, p27, E2F1, and TERT (1). CTCF also binds to and is required for the enhancer-blocking activity of all known insulator elements and ICRs, including the H19/IgF2, Prader-Willi/Angelman syndrome, and Inactive X-Specific Transcript (XIST) anti-sense loci (5-7). CTCF DNA-binding is sensitive to DNA methylation, a mark that determines selection of the imprinted allele (maternal vs. paternal) (1). The various functions of CTCF are regulated by at least two different post-translational modifications. Poly(ADP-ribosyl)ation of CTCF is required for insulator function (8). Phosphorylation of Ser612 by protein kinase CK2 facilitates a switch of CTCF from a transcriptional repressor to an activator at the c-myc promoter (9). CTCF mutations or deletions have been found in many breast, prostate, and Wilms tumors (10,11). Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF (3). In cells expressing BORIS, promoters of X-linked cancer-testis antigens like MAGE-1A are demethylated and activated, but methylated and inactive in CTCF-expressing somatic cells (12). Like other testis specific proteins, BORIS is abnormally expressed in different cancers, such as breast cancer, and has a greater affinity than CTCF for DNA binding sites, detracting from CTCF's potential tumor suppressing activity (1,3,13,14).

Product Categories/Family for anti-CTCF antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 1706179
• NCBI GeneID: 10664
• UniProt Accession #: P49711; Q53XI7; Q59EL8; B5MC38
• Molecular Weight: 727
• NCBI Official Full Name: Transcriptional repressor CTCF
• NCBI Official Synonym Full Names: CCCTC-binding factor (zinc finger protein)
• NCBI Official Symbol: CTCF
• NCBI Official Synonym Symbols: MRD21
• NCBI Protein Information: transcriptional repressor CTCF; CTCFL paralog; 11-zinc finger protein; 11 zinc finger transcriptional repressor
• UniProt Protein Name: Transcriptional repressor CTCF
• Protein Family: Transcriptional repressor
• UniProt Gene Name: CTCF
• UniProt Entry Name: CTCF_HUMAN

NCBI Description

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.

Protein type: Tumor suppressor; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 16q21-q22.3

Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein binding; zinc ion binding; chromatin insulator sequence binding; sequence-specific DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: genetic imprinting; maintenance of DNA methylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 21