Chromatin Immunoprecipitation (ChIP)
(CTBP2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse liver and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a regions of chromosome 11. You can view the complete data set in the UCSC Genome Browser)
Rabbit anti-Human, Mouse CTBP2 Polyclonal Antibody | anti-CTBP2 antibody
CTBP2 antibody (pAb)
ChIP-Seq: 4ug each
ChIP: 10ul per ChIP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Chromatin Immunoprecipitation (ChIP)
(CTBP2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse liver and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a regions of chromosome 11. You can view the complete data set in the UCSC Genome Browser)
Chromatin Immunoprecipitation (ChIP)
(CTBP2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse liver and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a regions of chromosome 11. You can view the complete data set in the UCSC Genome Browser)
Western Blot (WB)
(CTBP2 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (10 ug) probed with CTBP2 antibody (1:1000).)
Western Blot (WB)
(CTBP2 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (10 ug) probed with CTBP2 antibody (1:1000).)
Background: The CTBP2 (C-terminal binding protein 2) gene encodes a protein that is a corepressor targeting diverse transcription regulators. It functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses. Isoform 2 is also phosphorylated upon DNA damage, probably by ATM or ATR at Thr179; Ser181 and Ser185. Phosphorylation by HIPK2 on Ser428 induces proteasomal degradation.
NCBI and Uniprot Product Information
Uniprot Description
CTBP2: Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: transcriptional repressor complex; synapse; cell junction; nucleus
Molecular Function: protein binding; retinoic acid receptor binding; transcription coactivator activity; chromatin binding; NAD binding; transcription corepressor activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; white fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of retinoic acid receptor signaling pathway; viral genome replication; negative regulation of transcription, DNA-dependent
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Product Notes
The CTBP2 ctbp2 (Catalog #AAA388831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CTBP2 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CTBP2 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP-Seq: 4ug each ChIP: 10ul per ChIP WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the CTBP2 ctbp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTBP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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