Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hela and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)
Rabbit anti-Human CPSF4 Polyclonal Antibody | anti-CPSF4 antibody
CPSF4 Antibody
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hela and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hela and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)
NCBI and Uniprot Product Information
NCBI Description
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CPSF4: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). Belongs to the CPSF4/YTH1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA processing; Mitochondrial
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex
Molecular Function: zinc ion binding
Biological Process: modification by virus of host cellular process; virus-host interaction; modification by virus of host mRNA processing; viral infectious cycle; mRNA processing
Research Articles on CPSF4
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Product Notes
The CPSF4 cpsf4 (Catalog #AAA9414415) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CPSF4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPSF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500-1:2000. Researchers should empirically determine the suitability of the CPSF4 cpsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPSF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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