Mouse anti-Human ADAM17 / TACE Monoclonal Antibody | anti-ADAM17 antibody

Mouse Monoclonal [clone 1F6] (IgG2b,k) to Human ADAM17 / TACE

Cat. Num. AAA246540

• Gene Names: ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ADAM17 / TACE; Monoclonal Antibody; Mouse Monoclonal [clone 1F6] (IgG2b; k) to Human ADAM17 / TACE; Anti-ADAM17 / TACE Antibody (clone 1F6) IHC-plus; CD156B; CD156b antigen; CSVP; ADAM 17; NISBD; TNF-alpha converting enzyme; TNF-alpha convertase; TNF-alpha-converting enzyme; Snake venom-like protease; TACE; Human ADAM17; anti-ADAM17 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1F6
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 824

• Applicable Applications for anti-ADAM17 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), Proximity Ligation Assay
• Application Notes: ELISA, IHC-P (5 ug/ml), PLA, WB (1:500 - 1:1000); Usage: Western blot performed with the recombinant protein used as the immunogen. In situ Proximity Ligation Assay (Cell).
• Target Species: Human
• Immunogen Description: ADAM17 (NP_003174, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: ADAM17 / TACE antibody was raised against aDAM17 (NP_003174, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-ADAM17 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ADAM17 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ADAM17 is approximately 3 ng/ml as a capture antibody.)

Proximity Ligation Analysis

(Proximity Ligation Analysis (PLA) of protein-protein interactions between TGFA and ADAM17. HeLa cells were stained with anti-TGFA rabbit purified polyclonal 1:1200 and anti-ADAM17 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.)

Related Product Information for anti-ADAM17 antibody

Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately.

Product Categories/Family for anti-ADAM17 antibody

Family: Protease; Subfamily: Metallopeptidase M12B

NCBI and Uniprot Product Information

• NCBI GI #: 73747889
• NCBI GeneID: 6868
• NCBI Accession #: NP_003174.3
• NCBI GenBank Nucleotide #: NM_003183.4
• UniProt Accession #: P78536; O60226
• Molecular Weight: 78,543 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 17
• NCBI Official Symbol: ADAM17
• NCBI Official Synonym Symbols: CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM17
• UniProt Synonym Gene Names: CSVP; TACE; ADAM 17
• UniProt Entry Name: ADA17_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.24.86; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft

Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; neutrophil mediated immunity; T cell differentiation in the thymus; cell motility involved in cell locomotion; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; response to drug; spleen development; epidermal growth factor receptor signaling pathway; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; wound healing, spreading of epidermal cells

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1