Rabbit anti-Human CPSF4 Polyclonal Antibody | anti-CPSF4 antibody

CPSF4, ID (CPSF4, CPSF30, NAR, NEB1, Cleavage and polyadenylation specificity factor subunit 4, Cleavage and polyadenylation specificity factor 30kD subunit, NS1 effector domain-binding protein 1, No arches homolog) (Biotin)

Cat. Num. AAA6288430

• Gene Names: CPSF4; NAR; NEB1; CPSF30
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPSF4; Polyclonal Antibody; ID (CPSF4; CPSF30; NAR; NEB1; Cleavage and polyadenylation specificity factor subunit 4; Cleavage and polyadenylation specificity factor 30kD subunit; NS1 effector domain-binding protein 1; No arches homolog) (Biotin); No arches homolog; anti-CPSF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CPSF4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CPSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102~132 amino acids from the Central region of human CPSF4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPSF4 Antibody (Center) in Jurkat cell line lysates (35ug/lane).CPSF4 (arrow) was detected using the purified Pab.)

Related Product Information for anti-CPSF4 antibody

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

Product Categories/Family for anti-CPSF4 antibody

Antibodies; Binding Proteins

References

Twu, K.Y., et al. J. Virol. 81(15):8112-8121(2007). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Kaufmann, I., et al. EMBO J. 23(3):616-626(2004). de Vries, H., et al. EMBO J. 19(21):5895-5904(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 125987603
• NCBI GeneID: 10898
• NCBI Accession #: NP_001075028.1
• NCBI GenBank Nucleotide #: NM_001081559.1
• UniProt Accession #: O95639; Q6FGE6; Q86TF8; Q9BTW6; D6W5S8
• Molecular Weight: 27,419 Da
• NCBI Official Full Name: cleavage and polyadenylation specificity factor subunit 4 isoform 2
• NCBI Official Synonym Full Names: cleavage and polyadenylation specific factor 4, 30kDa
• NCBI Official Symbol: CPSF4
• NCBI Official Synonym Symbols: NAR; NEB1; CPSF30
• NCBI Protein Information: cleavage and polyadenylation specificity factor subunit 4; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; cleavage and polyadenylation specificity factor 30 kDa subunit; cleavage-polyadenylation specificity factor, 30kD; neb-1; no arches homo
• UniProt Protein Name: Cleavage and polyadenylation specificity factor subunit 4
• Protein Family: Cleavage and polyadenylation specificity factor
• UniProt Gene Name: CPSF4
• UniProt Synonym Gene Names: CPSF30; NAR; NEB1; CPSF 30 kDa subunit; Neb-1
• UniProt Entry Name: CPSF4_HUMAN

NCBI Description

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPSF4: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). Belongs to the CPSF4/YTH1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Mitochondrial

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex

Molecular Function: zinc ion binding

Biological Process: modification by virus of host cellular process; virus-host interaction; modification by virus of host mRNA processing; viral infectious cycle; mRNA processing