Western Blot (WB)
(Western blot analysis of extracts of Mouse pancreas, using CPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Rabbit anti-Mouse, Rat CPA3 Polyclonal Antibody | anti-CPA3 antibody
CPA3 Rabbit pAb
Gene Names
CPA3; MC-CPA
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
CPA3; Polyclonal Antibody; CPA3 Rabbit pAb; MC-CPA; anti-CPA3 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
IPGRHSYAKYNNWEKIVAWTEKMMDKYPEMVSRIKIGSTVEDNPLYVLKIGEKNERRKAIFTDCGIHAREWVSPAFCQWFVYQATKTYGRNKIMTKLLDRMNFYILPVFNVDGYIWSWTKNRMWRKNRSKNQNSKCIGTDLNRNFNASWNSIPNTNDPCADNYRGSAPESEKETKAVTNFIRSHLNEIKVYITFHSYSQMLLFPYGYTSKLPPNHEDLAKVAKIGTDVLSTRYETRYIYGPIESTIYPISGSSLDWAYDLGIKHTFAFELRDKGKFGFLLPESRIKPTCRETMLAVKFIAKYILKHTS
Applicable Applications for anti-CPA3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 110-417 of human CPA3 (NP_001861.2).
Cellular Location
Cytoplasmic vesicle, secretory vesicle
Positive Samples
Mouse pancreas, Rat pancreas
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of Mouse pancreas, using CPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse pancreas, using CPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Western Blot (WB)
(Western blot analysis of extracts of Rat pancreas, using CPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of Rat pancreas, using CPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-CPA3 antibody
Background: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients.
Product Categories/Family for anti-CPA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,670 Da
NCBI Official Full Name
mast cell carboxypeptidase A
NCBI Official Synonym Full Names
carboxypeptidase A3 (mast cell)
NCBI Official Symbol
CPA3
NCBI Official Synonym Symbols
MC-CPA
NCBI Protein Information
mast cell carboxypeptidase A; tissue carboxypeptidase A
UniProt Protein Name
Mast cell carboxypeptidase A
Protein Family
UniProt Gene Name
CPA3
UniProt Synonym Gene Names
MC-CPA
UniProt Entry Name
CBPA3_HUMAN
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Product Notes
The CPA3 cpa3 (Catalog #AAA9142083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CPA3 Rabbit pAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CPA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CPA3 cpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IPGRHSYAKY NNWEKIVAWT EKMMDKYPEM VSRIKIGSTV EDNPLYVLKI GEKNERRKAI FTDCGIHARE WVSPAFCQWF VYQATKTYGR NKIMTKLLDR MNFYILPVFN VDGYIWSWTK NRMWRKNRSK NQNSKCIGTD LNRNFNASWN SIPNTNDPCA DNYRGSAPES EKETKAVTNF IRSHLNEIKV YITFHSYSQM LLFPYGYTSK LPPNHEDLAK VAKIGTDVLS TRYETRYIYG PIESTIYPIS GSSLDWAYDL GIKHTFAFEL RDKGKFGFLL PESRIKPTCR ETMLAVKFIA KYILKHTS. It is sometimes possible for the material contained within the vial of "CPA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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