Goat FHL3/SLIM2 Polyclonal Antibody | anti-FHL3 antibody

Goat anti-FHL3/SLIM2 Antibody

Cat. Num. AAA421896

• Gene Names: FHL3; SLIM2
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FHL3/SLIM2; Polyclonal Antibody; Goat anti-FHL3/SLIM2 Antibody; FHL3; SLIM2; four and a half LIM domains 3; LIM-only protein FHL3; MGC19547; MGC23614; MGC8696; FHL3 antibody; SLIM2 antibody; four and a half LIM domains 3 antibody; LIM-only protein FHL3 antibody; MGC19547 antibody; MGC23614 antibody; MGC8696 antibody; FHL3 / SLIM2; anti-FHL3 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DQVLCQGCSQAGP
• Sequence Length: 280

• Applicable Applications for anti-FHL3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Approx 30kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 31.2kDa according to NP_004459.2). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence DQVLCQGCSQAGP, from the C Terminus of the protein sequence according to NP_004459.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 54112385
• NCBI GeneID: 2275
• NCBI Accession #: NP_004459.2
• NCBI GenBank Nucleotide #: NM_004468.4
• Molecular Weight: 31,192 Da
• NCBI Official Full Name: four and a half LIM domains protein 3 isoform 1
• NCBI Official Synonym Full Names: four and a half LIM domains 3
• NCBI Official Symbol: FHL3
• NCBI Official Synonym Symbols: SLIM2
• NCBI Protein Information: four and a half LIM domains protein 3
• UniProt Protein Name: Four and a half LIM domains protein 3
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL3
• UniProt Synonym Gene Names: SLIM2; FHL-3; SLIM-2
• UniProt Entry Name: FHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

FHL3:

Protein type: Actin-binding; Contractile

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: focal adhesion; nucleus; stress fiber; Z disc

Molecular Function: actin binding; protein binding; zinc ion binding

Biological Process: actin cytoskeleton organization and biogenesis; muscle development