Rabbit Cox-4 Polyclonal Antibody | anti-Cox-4 antibody

Cox-4 (A37) Polyclonal Antibody

Cat. Num. AAA3002254

• Gene Names: COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cox-4; Polyclonal Antibody; Cox-4 (A37) Polyclonal Antibody; Cytochrome c oxidase subunit 4 isoform 1; mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; COX4I1; COX4; anti-Cox-4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cox-4 (A37) polyclonal antibody detects endogenous levels of Cox-4 protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 169

• Applicable Applications for anti-Cox-4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Cox-4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cox-4 (A37) polyclonal antibody at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:LO2 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug) Lane6:C6 whole cell lysate (40ug) Lane7:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cox-4 (A37) pAb in paraffin-embedded human colon carcinoma tissue.)

Related Product Information for anti-Cox-4 antibody

Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.

NCBI and Uniprot Product Information

• NCBI GI #: 4502981
• NCBI GeneID: 1327
• NCBI Accession #: NP_001852.1
• NCBI GenBank Nucleotide #: NP_001852.1
• UniProt Accession #: P13073
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit 4I1
• NCBI Official Symbol: COX4I1
• NCBI Official Synonym Symbols: COX4; COXIV; COX4-1; COXIV-1; COX IV-1
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Gene Name: COX4I1
• UniProt Synonym Gene Names: COX4; COX IV-1
• UniProt Entry Name: COX41_HUMAN

NCBI Description

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

COX4I1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Ubiquitous. Belongs to the cytochrome c oxidase IV family.

Protein type: Oxidoreductase; Mitochondrial; EC 1.9.3.1; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; nucleus

Molecular Function: protein binding; cytochrome-c oxidase activity

Biological Process: cellular metabolic process; generation of precursor metabolites and energy; transmembrane transport; response to nutrient