Western Blot (WB)
(Western Blot analysis of COX IV on different lysates using anti-COX IV antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Rat heart)
Rabbit COX IV Polyclonal Antibody | anti-COXIV antibody
COX IV Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of COX IV on different lysates using anti-COX IV antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Rat heart)
Western Blot (WB)
(Western Blot analysis of COX IV on different lysates using anti-COX IV antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Rat heart)
Immunocytochemistry (ICC)
(ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Uniprot Description
COX4I1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Ubiquitous. Belongs to the cytochrome c oxidase IV family.
Protein type: Oxidoreductase; Mitochondrial; EC 1.9.3.1; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation
Chromosomal Location of Human Ortholog: 16q24.1
Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; nucleus
Molecular Function: protein binding; cytochrome-c oxidase activity
Biological Process: cellular metabolic process; generation of precursor metabolites and energy; transmembrane transport; response to nutrient
Research Articles on COXIV
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Product Notes
The COXIV cox4i1 (Catalog #AAA3012502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COX IV Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's COX IV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:5,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the COXIV cox4i1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX IV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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