Rabbit anti-Human COX4I2 Polyclonal Antibody | anti-COX4I2 antibody

COX4I2 Polyclonal Antibody

Cat. Num. AAA3007490

• Gene Names: COX4I2; COX4; COX4B; COX4-2; COX4L2; COXIV-2; dJ857M17.2
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >97% (by SDS-PAGE).
• Synonyms: COX4I2; Polyclonal Antibody; COX4I2 Polyclonal Antibody; Cytochrome c oxidase subunit 4 isoform 2; mitochondrial; Cytochrome c oxidase subunit IV isoform 2; COX IV-2; anti-COX4I2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: COX4I2 polyclonal antibody detects endogenous levels of COX4I2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >97% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 171

• Applicable Applications for anti-COX4I2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 77-121 of Human COX4I2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of COX4I2 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:Hcc827 whole cell lysate (40ug) Lane3:The lung tissue lysate of Fetal Mouse (40ug) Lane4:The lung tissue lysate of Fetal Rat (40ug))

Related Product Information for anti-COX4I2 antibody

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta. Two isoforms exist for COX4, namely COX4I1 and COX4I2, and they are encoded by two different genes. COX4I2 (Cytochrome c oxidase subunit 4 isoform 2), also known as COX4B, COX4-2, COX4L2 or COXIV-2, is the less common isoform and is incorporated into the COX apoenzyme under hypoxic conditions to optimize COX activity.

NCBI and Uniprot Product Information

• NCBI GI #: 17999526
• NCBI GeneID: 84701
• NCBI Accession #: NP_115998.2
• NCBI GenBank Nucleotide #: NP_115998.2
• UniProt Accession #: Q96KJ9
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit 4I2
• NCBI Official Symbol: COX4I2
• NCBI Official Synonym Symbols: COX4; COX4B; COX4-2; COX4L2; COXIV-2; dJ857M17.2
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• UniProt Gene Name: COX4I2
• UniProt Synonym Gene Names: COX4L2; COX IV-2
• UniProt Entry Name: COX42_HUMAN

NCBI Description

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

COX4I2: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Defects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH). Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth. Belongs to the cytochrome c oxidase IV family.

Protein type: Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: mitochondrial respiratory chain complex IV

Molecular Function: cytochrome-c oxidase activity

Biological Process: generation of precursor metabolites and energy; cellular respiration

Disease: Exocrine Pancreatic Insufficiency, Dyserythropoietic Anemia, And Calvarial Hyperostosis