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ELISA (EIA) (Figure 1 ELISA Validation Coating Antigen: immunogen peptide, 9157P, 10ug/mL, incubate at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. was detected by HRP-conjugated streptavidin at 1:5,000 and 9157 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000, incubate at RT for 1 hr.)

Rabbit COVID 19 Membrane Coronavirus Polyclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 (COVID-19) Membrane Antibody (biotin)

Reactivity
Virus
Predicted reactivity based on immunogen sequence: SARS-CoV Matrix protein: (100%)
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography
Synonyms
COVID 19 Membrane Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) Membrane Antibody (biotin); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; MembraneSARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein; SARS Matrix protein; Membrane protein; E1 glycoprotein; M protein; anti-COVID-19 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Virus
Predicted reactivity based on immunogen sequence: SARS-CoV Matrix protein: (100%)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography
Form/Format
Liquid; SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA)
Immunogen
Anti-SARS-CoV-2 (COVID-19) Membrane antibody (biotin) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Membrane protein.

The immunogen is located between 130-180 amino acids of the SARS-CoV-2 (COVID-19) Membrane protein.
Conjugation
Biotin
Protein GI#
1800455127
Preparation and Storage
SARS-CoV-2 (COVID-19) Membrane antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA)

(Figure 1 ELISA Validation Coating Antigen: immunogen peptide, 9157P, 10ug/mL, incubate at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. was detected by HRP-conjugated streptavidin at 1:5,000 and 9157 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000, incubate at RT for 1 hr.)

ELISA (EIA) (Figure 1 ELISA Validation Coating Antigen: immunogen peptide, 9157P, 10ug/mL, incubate at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. was detected by HRP-conjugated streptavidin at 1:5,000 and 9157 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000, incubate at RT for 1 hr.)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019-20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2).The membrane (M) protein or matrix protein is the most abundant structural protein and defines the shape of the viral envelope (3). It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein. It involves in organization of the nucleoprotein inside, which includes many copies of the N (nucleocapsid) protein bound to the genomic RNA. The M protein holds dominant cellular immunogenicity and has been determined as a protective antigen in humoral responses, which suggests it would serve as a potential target in vaccine design (4).
Product Categories/Family for anti-COVID-19 antibody
References
Zhu et al. N Engl J Med. 2020 Feb 20;382(8):727-733.
Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575
Neuman et al. J Struct Biol. 2011;174(1):11-22.
Liu et al. J Infect Dis. 2010;202(8):1171-80.

NCBI and Uniprot Product Information

NCBI GeneID

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Product Notes

The COVID-19 (Catalog #AAA154814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Membrane Antibody (biotin) reacts with Virus Predicted reactivity based on immunogen sequence: SARS-CoV Matrix protein: (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Membrane Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Membrane Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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