Rabbit Coronin 1a/TACO/CORO1A Polyclonal Antibody | anti-CORO1A antibody
Anti-Coronin 1a/TACO/CORO1A Antibody
IHC-P: 1-2ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: rat spleen tissue lysatesLane 3: rat thymus tissue lysatesLane 4: mouse spleen tissue lysatesLane 5: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Coronin 1a/TACO/CORO1A antigen affinity purified polyclonal antibody (Catalog # MBS1753651) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Coronin 1a/TACO/CORO1A at approximately 57KD. The expected band size for Coronin 1a/TACO/CORO1A is at 57KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 7. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 8. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 9. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Flow Cytometry (FC/FACS)
(Figure 10. Flow Cytometry analysis of THP-1 cells using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Overlay histogram showing THP-1 cells stained with MBS1753651 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (MBS1753651).Overlay histogram showing U937 cells stained with MBS1753651 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (MBS1753651, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Foger, N., Rangell, L., Danilenko, D. M., Chan, A. C. Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis. Science 313: 839-842, 2006.
3. Haraldsson, M. K., Louis-Dit-Sully, C. A., Lawson, B. R., Sternik, G., Santiago-Raber, M. -L., Gascoigne, N. R. J., Theofilopoulos, A. N., Kono, D. H. The lupus-related Lmb3 locus contains a disease-suppressing coronin-1A gene mutation. Immunity 28: 40-51, 2008.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]
Uniprot Description
coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cortical actin cytoskeleton; protein complex; early endosome; immunological synapse; actin filament; phagocytic vesicle; intercellular junction; phagocytic vesicle membrane; membrane; axon; lamellipodium; cytoplasm; plasma membrane; nucleus; phagocytic cup
Molecular Function: actin filament binding; protein C-terminus binding; protein binding; protein homodimerization activity; cytoskeletal protein binding; phosphoinositide 3-kinase binding
Biological Process: negative regulation of vesicle fusion; leukocyte chemotaxis; actin filament organization; regulation of release of sequestered calcium ion into cytosol; cell-substrate adhesion; phagocytosis; uropod organization and biogenesis; phagolysosome formation; regulation of cell shape; regulation of actin filament polymerization; positive chemotaxis; homeostasis of number of cells within a tissue; calcium ion transport; negative regulation of actin nucleation; innate immune response; positive regulation of T cell proliferation; T cell homeostasis; negative regulation of neuron apoptosis; cell motility; actin cytoskeleton organization and biogenesis; positive regulation of cell migration
Disease: Immunodeficiency 8
Research Articles on CORO1A
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Product Notes
The CORO1A coro1a (Catalog #AAA1753651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Coronin 1a/TACO/CORO1A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Coronin 1a/TACO/CORO1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| IHC-P: 1-2ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the CORO1A coro1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coronin 1a/TACO/CORO1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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