Bovine Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit | PIGR elisa kit

Cattle Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit

Cat. Num. AAA2702890

• Reactivity: Bovine
• Synonyms: Polymeric Immunoglobulin Receptor (PIGR); Cattle Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit; SC; Secretory Component; Hepatocellular carcinoma-associated protein TB6; PIGR elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Polymeric Immunoglobulin Receptor (PIGR).; No significant cross-reactivity or interference between Polymeric Immunoglobulin Receptor (PIGR) and analogues was observed.
• Sequence Length: 764

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.238ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polymeric Immunoglobulin Receptor (PIGR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polymeric Immunoglobulin Receptor (PIGR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIGR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymeric Immunoglobulin Receptor (PIGR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymeric Immunoglobulin Receptor (PIGR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymeric Immunoglobulin Receptor (PIGR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymeric Immunoglobulin Receptor (PIGR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31377806
• NCBI GeneID: 5284
• NCBI Accession #: NP_002635.2
• NCBI GenBank Nucleotide #: NM_002644.3
• UniProt Accession #: P01833; Q68D81; Q8IZY7
• Molecular Weight: 83,284 Da
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: PIGR
• NCBI Protein Information: polymeric immunoglobulin receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• Protein Family: Polymeric immunoglobulin receptor
• UniProt Gene Name: PIGR
• UniProt Synonym Gene Names: PIgR; Poly-Ig receptor

NCBI Description

This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.