Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat NALP2/PAN1 Polyclonal Antibody | anti-NLRP2 antibody
Goat anti-NALP2/PAN1 Antibody
Western Blot: In transfected HEK293 transiently expressing full-length Human NLRP2 (myc and DYKDDDDK tagged), a band of approx.150kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
NALP proteins, such as NALP2, are characterized by an N-terminal pyrin (MIM 608107) domain (PYD) and are involved in the activation of caspase-1 (CASP1; MIM 147678) by Toll-like receptors (see TLR4; MIM 603030). They may also be involved in protein complexes that activate proinflammatory caspases (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM, Mar 2008]
Uniprot Description
NLRP2: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nuclear chromosome, telomeric region
Molecular Function: ATP binding; protein binding; Pyrin domain binding
Biological Process: inflammatory response; innate immune response; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion
Research Articles on NLRP2
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Product Notes
The NLRP2 nlrp2 (Catalog #AAA422317) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NALP2/PAN1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's NALP2/PAN1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: In transfected HEK293 transiently expressing full-length Human NLRP2 (myc and DYKDDDDK tagged), a band of approx.150kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml. Researchers should empirically determine the suitability of the NLRP2 nlrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KNPQLIIDTE KHHP. It is sometimes possible for the material contained within the vial of "NALP2/PAN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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