Goat anti-Rat Connexin 43 Polyclonal Antibody | anti-Gja1 antibody

Connexin 43 (Gap junction alpha-1 protein, Cx43, Connexin43, Connexin-43, Gap junction 43kD heart protein, GJA1)

Cat. Num. AAA628391

• Gene Names: Gja1; Cx43; Npm1; Cnx43; Gja-1; AU042049; AW546267; Cx43alpha1; connexin43
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Connexin 43; Polyclonal Antibody; Connexin 43 (Gap junction alpha-1 protein; Cx43; Connexin43; Connexin-43; Gap junction 43kD heart protein; GJA1); Anti -Connexin 43 (Gap junction alpha-1 protein; anti-Gja1 antibody

• Host: Goat
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat GJA1. Sequence Homology: human, canine, porcine.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

• Applicable Applications for anti-Gja1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot.; Dilution: ELISA: 1:64,000
• Immunogen: Synthetic peptide corresponding to C-QPFDFPDDNQNSKK, from the internal region of rat GJA1(NP_000156.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-Gja1 antibody

Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1kD. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junctions play an important role for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junctions may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane domains: 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N- and C-termini are predicted to be cytoplasmic.

Product Categories/Family for anti-Gja1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 50510
• NCBI GeneID: 14609
• UniProt Accession #: P23242; Q3UTR0; Q544I7; Q6T9D4; Q7TMQ1; Q8C4N2; Q8CE05; Q9CT20
• Molecular Weight: 43,004 Da
• NCBI Official Full Name: connexin 43
• NCBI Official Synonym Full Names: gap junction protein, alpha 1
• NCBI Official Symbol: Gja1
• NCBI Official Synonym Symbols: Cx43; Npm1; Cnx43; Gja-1; AU042049; AW546267; Cx43alpha1; connexin43
• NCBI Protein Information: gap junction alpha-1 protein; connexin 43; connexin-43; alpha 1 connexin; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1
• UniProt Protein Name: Gap junction alpha-1 protein
• Protein Family: Gap junction alpha-1 protein
• UniProt Gene Name: Gja1
• UniProt Synonym Gene Names: Cxn-43
• UniProt Entry Name: CXA1_MOUSE

Uniprot Description

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; focal adhesion; contractile fiber; lysosome; early endosome; integral to membrane; intermediate filament; intercellular junction; fascia adherens; cytosol; lipid raft; multivesicular body; connexon complex; mitochondrial outer membrane; membrane; late endosome; apical plasma membrane; cytoplasm; plasma membrane; gap junction; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; ion transmembrane transporter activity; beta-tubulin binding; transmembrane transporter activity; SH3 domain binding; gap junction channel activity; PDZ domain binding; receptor binding

Biological Process: lens development in camera-type eye; apoptosis; heart development; neuron migration; milk ejection; positive regulation of vasodilation; signal transduction; regulation of heart contraction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; negative regulation of cardiac muscle cell proliferation; positive regulation of glomerular filtration; positive regulation of striated muscle development; cell communication; heart looping; transmembrane transport; adult heart development; ATP transport; intercellular junction assembly and maintenance; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; epithelial cell maturation; in utero embryonic development; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of protein catabolic process; positive regulation of vasoconstriction; blood vessel morphogenesis; embryonic heart tube development; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH