SDS-Page
Phospholipase A2 Recombinant Protein | PLA2G4D recombinant protein
Recombinant Phospholipase A2, Group IVD (PLA2G4D)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 90%
Synonyms
Phospholipase A2; Recombinant Phospholipase A2; Group IVD (PLA2G4D); cPLA2delta; Cytosolic phospholipase A2 delta; PLA2G4D recombinant protein
Host
E Coli
Purity/Purification
> 90%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
PP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQLSTAPGMKFK TKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTEDDICFKVLYD ISEVLPGKLL
Sequence Length
145
Applicable Applications for PLA2G4D recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Predicted Molecular Mass
42.8kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Pro9~Leu120
Organism Species
Human
Tag
two N-terminal Tags, His-tag and GST-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,002 Da
NCBI Official Full Name
phospholipase A2 preproprotein
NCBI Official Symbol
PLA2G1B
NCBI Protein Information
phospholipase A2
UniProt Protein Name
Phospholipase A2
Protein Family
UniProt Gene Name
PLA2G1B
UniProt Entry Name
PA21B_BOVIN
Uniprot Description
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
Research Articles on PLA2G4D
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Product Notes
The PLA2G4D pla2g1b (Catalog #AAA2032985) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospholipase A2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PLA2G4D pla2g1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQ LSTAPGMKFK TKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTE DDICFKVLYD ISEVLPGKLL. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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