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Western Blot (WB) (Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Connexin 43. Arrow indicates protein Connexin 43 (~43kD) using C7857.)

Mouse Connexin 43 Monoclonal Antibody | anti-Cx43 antibody

Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1) (FITC)

Gene Names
Gja1; Cx43; Npm1; Cnx43; Gja-1; AU042049; AW546267; Cx43alpha1; connexin43
Reactivity
Canine, Human, Mouse, Porcine, Rat
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography
Synonyms
Connexin 43; Monoclonal Antibody; Connexin 43 (Cx43; Gap Junction Alpha 1 Protein; CxA-1) (FITC); anti-Cx43 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Canine, Human, Mouse, Porcine, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q250
Specificity
Recognizes mouse Connexin 43, Mr 43-47kD. Species Crossreactivity: porcine, rat, human and canine. Species Sequence Homology: bovine. No known homology with other connexins.
Purity/Purification
Purified by Protein A Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-Cx43 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)
Immunogen
Synthetic peptide corresponding to aa252-270 at the C-terminal of the mouse connexin 43 sequence.
Conjugate
FITC
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Connexin 43. Arrow indicates protein Connexin 43 (~43kD) using C7857.)

Western Blot (WB) (Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Connexin 43. Arrow indicates protein Connexin 43 (~43kD) using C7857.)

Western Blot (WB)

(Western Blot Analysis: Respresentative image from a previous lot. Mouse brain (Lane1) and rat brain (Lane2) were resolved by electrophoresis transfered and probed with C7857. Arrow indicates protein Connexin 43 (~43kD).)

Western Blot (WB) (Western Blot Analysis: Respresentative image from a previous lot. Mouse brain (Lane1) and rat brain (Lane2) were resolved by electrophoresis transfered and probed with C7857. Arrow indicates protein Connexin 43 (~43kD).)
Product Categories/Family for anti-Cx43 antibody
References
1. Tansey, Erin E, et al. (2006). Ann. Thorac. Surg. 82: 1472-1479. 2. Arita, Ken, et al. (2006). Am. J. Pathol. 169: 416-423.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
43,004 Da
NCBI Official Full Name
connexin 43
NCBI Official Synonym Full Names
gap junction protein, alpha 1
NCBI Official Symbol
Gja1
NCBI Official Synonym Symbols
Cx43; Npm1; Cnx43; Gja-1; AU042049; AW546267; Cx43alpha1; connexin43
NCBI Protein Information
gap junction alpha-1 protein
UniProt Protein Name
Gap junction alpha-1 protein
UniProt Gene Name
Gja1
UniProt Synonym Gene Names
Cxn-43; Cx43

Uniprot Description

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed:15181016). Plays an essential role in gap junction communication in the ventricles (PubMed:26403541).

Research Articles on Cx43

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Product Notes

The Cx43 gja1 (Catalog #AAA6243237) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1) (FITC) reacts with Canine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 43 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Cx43 gja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 43, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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