Rabbit CONNEXIN 43 Polyclonal Antibody | anti-Gja1 antibody

RABBIT ANTI CONNEXIN 43 (pSer368)

Cat. Num. AAA223268

• Gene Names: Gja1; Cx43
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Guinea Pig (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
• Applications: Western Blot
• Synonyms: CONNEXIN 43; Polyclonal Antibody; RABBIT ANTI CONNEXIN 43 (pSer368); anti-Gja1 antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Guinea Pig (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 382

• Applicable Applications for anti-Gja1 antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band of approximately 43kDa in rat hippocampus cell lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368; Buffer Solution
• Antiserum Preparation: Antisera to rat connexin43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-Gja1 antibody

MBS223268 specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.

NCBI and Uniprot Product Information

• NCBI GI #: 6978896
• NCBI GeneID: 24392
• NCBI Accession #: NP_036699.1
• NCBI GenBank Nucleotide #: NM_012567.2
• UniProt Accession #: P08050; Q53ZE1
• Molecular Weight: 43,031 Da
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 1
• NCBI Official Symbol: Gja1
• NCBI Official Synonym Symbols: Cx43
• NCBI Protein Information: gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1
• UniProt Protein Name: Gap junction alpha-1 protein
• Protein Family: Gap junction alpha-1 protein
• UniProt Gene Name: Gja1
• UniProt Synonym Gene Names: Cxn-43; Cx43
• UniProt Entry Name: CXA1_RAT

NCBI Description

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

Uniprot Description

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Channel, misc.; Membrane protein, integral

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; focal adhesion; contractile fiber; lysosome; integral to plasma membrane; early endosome; intermediate filament; fascia adherens; intercellular junction; cytosol; lipid raft; Golgi membrane; connexon complex; multivesicular body; mitochondrial outer membrane; membrane; cytoplasm; apical plasma membrane; late endosome; plasma membrane; gap junction; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; ion transmembrane transporter activity; beta-tubulin binding; SH3 domain binding; gap junction channel activity; receptor binding; PDZ domain binding

Biological Process: lens development in camera-type eye; response to peptide hormone stimulus; apoptosis; heart development; neuron migration; milk ejection; positive regulation of vasodilation; signal transduction; regulation of heart contraction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; positive regulation of glomerular filtration; negative regulation of cardiac muscle cell proliferation; response to glucose stimulus; positive regulation of striated muscle development; cell communication; heart looping; transmembrane transport; ATP transport; adult heart development; intercellular junction assembly and maintenance; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; epithelial cell maturation; in utero embryonic development; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of vasoconstriction; positive regulation of protein catabolic process; embryonic heart tube development; blood vessel morphogenesis; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH