Rabbit Connexin 43 Polyclonal Antibody | anti-R361 antibody

Connexin 43 (R361) Polyclonal Antibody

Cat. Num. AAA3004061

• Gene Names: GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Connexin 43; Polyclonal Antibody; Connexin 43 (R361) Polyclonal Antibody; Gap junction alpha-1 protein; Connexin-43; Connexin43; Cx43; Cx-43; Gap junction 43 kDa heart protein; GJA1; GJAL; anti-R361 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Connexin 43 (R361) polyclonal antibody detects endogenous levels of Connexin 43 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 382

• Applicable Applications for anti-R361 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 330-380 of Human Connexin 43.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Connexin 43 (R361) pAb at 1:1000 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:The Testis tissue lysate of Rat (40ug) Lane5:The Brain tissue lysate of Mouse (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Connexin 43 (R361) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-R361 antibody

Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.

NCBI and Uniprot Product Information

• NCBI GI #: 4504001
• NCBI GeneID: 2697
• NCBI Accession #: NP_000156.1
• NCBI GenBank Nucleotide #: NP_000156.1
• UniProt Accession #: P17302
• Molecular Weight: ~ 43kDa
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein alpha 1
• NCBI Official Symbol: GJA1
• NCBI Official Synonym Symbols: HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
• NCBI Protein Information: gap junction alpha-1 protein
• UniProt Protein Name: Gap junction alpha-1 protein
• UniProt Gene Name: GJA1
• UniProt Synonym Gene Names: GJAL; Cx43
• UniProt Entry Name: CXA1_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]

Uniprot Description

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Protein type: Channel, misc.; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; focal adhesion; contractile fiber; lysosome; integral to plasma membrane; early endosome; intermediate filament; fascia adherens; cytosol; lipid raft; Golgi membrane; connexon complex; multivesicular body; mitochondrial outer membrane; apical plasma membrane; plasma membrane; gap junction; lateral plasma membrane

Molecular Function: signal transducer activity; protein binding; ion transmembrane transporter activity; beta-tubulin binding; SH3 domain binding; gap junction channel activity; PDZ domain binding; receptor binding

Biological Process: lens development in camera-type eye; response to peptide hormone stimulus; apoptosis; heart development; neuron migration; milk ejection; signal transduction; positive regulation of vasodilation; elevation of cytosolic calcium ion concentration; cell-cell signaling; muscle contraction; transport; positive regulation of glomerular filtration; negative regulation of cardiac muscle cell proliferation; response to glucose stimulus; positive regulation of striated muscle development; heart looping; ATP transport; adult heart development; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; in utero embryonic development; epithelial cell maturation; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of protein catabolic process; positive regulation of vasoconstriction; blood vessel morphogenesis; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH

Disease: Syndactyly, Type Iii; Craniometaphyseal Dysplasia, Autosomal Recessive; Palmoplantar Keratoderma And Congenital Alopecia 1; Atrioventricular Septal Defect 3; Oculodentodigital Dysplasia; Erythrokeratodermia Variabilis Et Progressiva; Oculodentodigital Dysplasia, Autosomal Recessive; Hypoplastic Left Heart Syndrome 1