Immunohistochemistry (IHC)
(Immunochemical staining of rat NPC2 in rat testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Rat NPC2 Polyclonal Antibody | anti-NPC2 antibody
Anti-NPC2 Antibody, Rabbit Polyclonal
Gene Names
Npc2; re1
Reactivity
Rat
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NPC2; Polyclonal Antibody; Anti-NPC2 Antibody; Rabbit Polyclonal; NPC2 Antibody; Rabbit PAb; Antigen Affinity Purified; Niemann-Pick disease; type C2; anti-NPC2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat NPC2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NPC2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Rat NPC2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat NPC2 (rR NPC2). NPC2 specific IgG was purified by Rat NPC2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of rat NPC2 in rat testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat NPC2 in rat testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat NPC2 in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat NPC2 in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Western Blot (WB)
(Anti-NPC2 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse lung tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-NPC2 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse lung tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-NPC2 antibody
Niemann-Pick Type C2 (NPC2) plays an important role in the regulation of intracellular cholesterol homeostasis via direct binding with free cholesterol. NPC2 is an intralysosomal protein that binds cholesterol in vitro. NPC2 is a small lysosomal glycoprotein that binds cholesterol with submicromolar affinity. Deficiency in NPC2 is the cause of Niemann-Pick type C2 disease, a fatal neurovisceral disorder characterized by the accumulation of cholesterol in lysosomes. Niemann-Pick disease, type C2 (NPC2) protein is one of the most abundant components of the epididymal fluid and contains a functional cholesterol-binding site that can transfer cholesterol between membranes, it has been suggested for years that NPC2 could be involved in the regulation of cholesterol levels in spermatozoa during epididymal maturation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,364 Da
NCBI Official Full Name
epididymal secretory protein 1
NCBI Official Synonym Full Names
NPC intracellular cholesterol transporter 2
NCBI Official Symbol
Npc2
NCBI Official Synonym Symbols
re1
NCBI Protein Information
epididymal secretory protein E1
Protein Family
NCBI Description
an epididymal secretory protein [RGD, Feb 2006]
Research Articles on NPC2
Similar Products
Product Notes
The NPC2 (Catalog #AAA8113070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NPC2 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the NPC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
