Human Cathepsin L PicoKine ELISA Kit | CTSL elisa kit

Human Cathepsin L PicoKine ELISA Kit

Cat. Num. AAA177633

• Gene Names: CTSL; MEP; CATL; CTSL1
• Reactivity: Human
• Synonyms: Cathepsin L PicoKine; Human Cathepsin L PicoKine ELISA Kit; Cathepsin L1; Cathepsin L1 light chain; CathepsinL; CATL; CATL1_HUMAN; CTSL; CTSL1; FLJ31037; Major excreted protein; MEP ; cathepsin L; CTSL elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Cathepsin L
• Sequence Length: 333

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human Cathepsin L in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, E113-V333
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human Cathepsin L standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin L standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|477|1541|2626|527|1712|3015; Standard deviation|27.7|92.46|128.7|32.15|114.7|168.84; CV(%)|5.8|6|4.9|6.1|6.7|5.6
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.056|0.112|0.169|0.276|0.459|0.845|1.484|2.081
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Cathepsin L PicoKine ELISA Kit standard curve )

Related Product Information for CTSL elisa kit

Principle of the assay: Human Cathepsin L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin L has been precoated onto 96-well plates. Standards(NSO, E113-V333) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin L amount of sample captured in plate.

Background: Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

NCBI and Uniprot Product Information

• NCBI GI #: 384081592
• NCBI GeneID: 1514
• NCBI Accession #: NP_001244900.1
• NCBI GenBank Nucleotide #: NM_001257971.1
• UniProt Accession #: P07711; Q6IAV1; Q96QJ0
• Molecular Weight: 37,564 Da
• NCBI Official Full Name: cathepsin L1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: CTSL
• NCBI Official Synonym Symbols: MEP; CATL; CTSL1
• NCBI Protein Information: cathepsin L1
• UniProt Protein Name: Cathepsin L1
• UniProt Gene Name: CTSL
• UniProt Synonym Gene Names: CTSL1; MEP
• UniProt Entry Name: CATL1_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Important for the overall degradation of proteins in lysosomes.