Rabbit anti-Mouse, Rat Connexin 36 Polyclonal Antibody
Connexin 36 (CX36)
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Connexin 36; Polyclonal Antibody; Connexin 36 (CX36); Anti -Connexin 36 (CX36); anti-Connexin 36 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat and mouse Cx36 protein (~36kD). Speci city was con rmed by Western Blot with lysates from rat retina and olfactory bulb. A homodimer may be observed. Species sequence homology: human.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-Connexin 36 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: ELISA: 0.1-1.0ug/ml
Immunohistochemistry: Frozen sections; Reactivity was con rmed with frozen sections from rat brain (stained: olfactory bulb and inferior olive), retina (stained: inner and outer plexiform layers, inner nuclear layer) and spinal cord (stained: gray matter).
Western Blot: 1ug/ml
Dilution: ELISA: 0.1-1.0ug/ml
Immunohistochemistry: Frozen sections; Reactivity was con rmed with frozen sections from rat brain (stained: olfactory bulb and inferior olive), retina (stained: inner and outer plexiform layers, inner nuclear layer) and spinal cord (stained: gray matter).
Western Blot: 1ug/ml
Immunogen
Peptide corresponding to a sequence located in the cytoplasmic loop between the second and third transmembrane domains of rat and mouse Connexin 36 (Cx36). This sequence differs from the human Cx36 protein by a single amino acid.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Connexin 36 antibody
The murine Cx36 gene encodes a protein of 321 amino acids most homologous to connexin 35 (6,7). Based on the presence of an intron within its coding region the Cx36 gene is suggested to form a new delta subclass of murine connexin genes (7). Cx36 is the first gap junction protein expressed predominantly in neuronal cells of the mammalian central nervous system. It is highly expressed in adult retina and is present in neurons of the inferior olive, the olfactory bulb, the CA3/CA4 hippocampal subfields and several brain-stem nuclei (6). Cx36 mRNA expression in brain increases gradually during fetal development until day 7 post-partum when its expression begins to decline (7). Biophysical measurements of gap junction channels formed by transfected and endogenous Cx36 indicate that they posess unique properties well suited for mediating flexible electrical and biochemical interactions between neurons (8).
Product Categories/Family for anti-Connexin 36 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
connexin 36
NCBI Description
This gene encodes a member of the connexin protein family. Connexins are gap junction proteins which are arranged in groups of 6 around a central pore to form a connexon, a component of the gap junction intercellular channel. The channels formed by this protein allow cationic molecule exchange between human beta cells and may function in the regulation of insulin secretion. [provided by RefSeq, Oct 2012]
Uniprot Description
GJD2: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Delta-type subfamily.
Protein type: Membrane protein, integral; Channel, misc.; Cell adhesion; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 15q14
Cellular Component: connexon complex; plasma membrane; integral to membrane
Molecular Function: gap junction channel activity
Biological Process: regulation of action potential; synaptic transmission; visual perception; transmembrane transport
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Product Notes
The Connexin 36 (Catalog #AAA614074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Connexin 36 (CX36) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 36 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Immunohistochemistry and Western Blot. Dilution: ELISA: 0.1-1.0ug/ml Immunohistochemistry: Frozen sections; Reactivity was con rmed with frozen sections from rat brain (stained: olfactory bulb and inferior olive), retina (stained: inner and outer plexiform layers, inner nuclear layer) and spinal cord (stained: gray matter). Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the Connexin 36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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