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Western Blot (WB) (Western Blot analysis of MBS631399 (1:2000) Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted Band: 36kD Blocking/Dilution Buffer: 5% NFDM/TBST)

Rabbit anti-Human Connexin 36 Polyclonal Antibody

Connexin 36, CT (GJA9, Gap Junction delta-2 Protein, Gap Junction alpha-9 Protein, Connexin-36, Cx36, GJD2)

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Connexin 36; Polyclonal Antibody; CT (GJA9; Gap Junction delta-2 Protein; Gap Junction alpha-9 Protein; Connexin-36; Cx36; GJD2); Anti -Connexin 36; anti-Connexin 36 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Connexin 36.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-Connexin 36 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Immunogen
Synthetic peptide corresponding to aa292-321 from the C-terminal region of human Connexin 36, conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS631399 (1:2000) Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted Band: 36kD Blocking/Dilution Buffer: 5% NFDM/TBST)

Western Blot (WB) (Western Blot analysis of MBS631399 (1:2000) Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted Band: 36kD Blocking/Dilution Buffer: 5% NFDM/TBST)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS631399, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS631399 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS631399, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS631399 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-Connexin 36 antibody
Connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.
Product Categories/Family for anti-Connexin 36 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
connexin 36

NCBI Description

This gene encodes a member of the connexin protein family. Connexins are gap junction proteins which are arranged in groups of 6 around a central pore to form a connexon, a component of the gap junction intercellular channel. The channels formed by this protein allow cationic molecule exchange between human beta cells and may function in the regulation of insulin secretion. [provided by RefSeq, Oct 2012]

Uniprot Description

GJD2: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Delta-type subfamily.

Protein type: Membrane protein, integral; Channel, misc.; Cell adhesion; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: connexon complex; plasma membrane; integral to membrane

Molecular Function: gap junction channel activity

Biological Process: regulation of action potential; synaptic transmission; visual perception; transmembrane transport

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Product Notes

The Connexin 36 (Catalog #AAA631399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Connexin 36, CT (GJA9, Gap Junction delta-2 Protein, Gap Junction alpha-9 Protein, Connexin-36, Cx36, GJD2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 36 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the Connexin 36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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