Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse CPLX2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Complexin 2 (CPLX2) Polyclonal Antibody | anti-CPLX2 antibody
Polyclonal Antibody to Complexin 2 (CPLX2)
Gene Names
Cplx2; 921-L; AI413745; AW492120
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Complexin 2 (CPLX2); Polyclonal Antibody; Polyclonal Antibody to Complexin 2 (CPLX2); anti-CPLX2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CPLX2. It has been selected for its ability to recognize CPLX2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-MDFVMKQALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA KHARMEAERE KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA IPAGCGDEEE EEEESILDTVLKYLPGPLQD MFKK
MGHHHHHHSGSEF-MDFVMKQALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA KHARMEAERE KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA IPAGCGDEEE EEEESILDTVLKYLPGPLQD MFKK
Sequence Length
114
Applicable Applications for anti-CPLX2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CPLX2 (Met1~Lys134) expressed in E.coli.
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076285
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse CPLX2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse CPLX2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,394 Da
NCBI Official Full Name
complexin-2
NCBI Official Synonym Full Names
complexin 2
NCBI Official Symbol
Cplx2
NCBI Official Synonym Symbols
921-L; AI413745; AW492120
NCBI Protein Information
complexin-2
UniProt Protein Name
Complexin-2
Protein Family
UniProt Gene Name
Cplx2
UniProt Synonym Gene Names
CPX II
Uniprot Description
CPLX2: Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis. Belongs to the complexin/synaphin family.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 13|13 B1
Cellular Component: cell soma; cytoplasm; dendrite; SNARE complex; synapse; terminal bouton
Molecular Function: calcium-dependent protein binding; SNARE binding; syntaxin binding; syntaxin-1 binding
Biological Process: cell differentiation; exocytosis; mast cell degranulation; nervous system development; neurotransmitter transport; positive regulation of synaptic plasticity; synaptic vesicle exocytosis; transport
Research Articles on CPLX2
Similar Products
Product Notes
The CPLX2 cplx2 (Catalog #AAA2003932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Complexin 2 (CPLX2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Complexin 2 (CPLX2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CPLX2 cplx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-MDFVMK QALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA KHARMEAERE KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA IPAGCGDEEE EEEESILDTV LKYLPGPLQD MFKK. It is sometimes possible for the material contained within the vial of "Complexin 2 (CPLX2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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