Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide
recombinant proteins, antibodies and ELISA kits to most target proteins.
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LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '13384'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
2.85 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '13384' and pd.language_id = 1
Query
Database
2.36 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '13384'
Query
Database
8.34 ms
UPDATE `products_description` SET `products_viewed` = products_viewed + 1
WHERE `products_id` = 13384
Database (5 total Queries, 5 of them unique across 2 Connections)
Time
Query String
1.98 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '13384'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
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JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
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⇄⧉specificity => string (189) "Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 18...
$value['specificity']
Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 183-197. Has been found to bind only to C. botulinum Toxin A cleaved SNAP-25 by ELISA and cell staining techniques.
⇄purity => string (28) "Product is sterile filtered."
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⇄form => string (19) "Supernatant, Liquid"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (71) "Upon receipt, store at -20 degree C. Avoid multiple freeze/thaw cycles."
⇄⧉app_notes => string (195) "Each laboratory should determine an optimum working titer for use in its par...
$value['app_notes']
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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⇄products_name_syn => string (69) "Monoclonal Antibody to Synaptosomal Protein 25 kDa (SNAP-25), cleaved"
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⇄products_gene_name => string (3) "N/A"
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⇄⧉products_description => string (96) "MAb to SNAP-25, Cleaved<br>Monoclonal Antibody to Synaptosomal Protein 25 kD...
$value['products_description']
MAb to SNAP-25, Cleaved<br>Monoclonal Antibody to Synaptosomal Protein 25 kDa (SNAP-25), Cleaved
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⇄products_related_diseases => string (3) "N/A"
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⇄products_categories => string (38) "Monoclonal Antibodies for Neuroscience"
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⇄products_search_terms => string (3) "N/A"
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⇄ncbi_symbol => string (3) "N/A"
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⇄ncbi_symbol_syn => string (3) "N/A"
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⇄ncbi_protein_info => string (3) "N/A"
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⇄ncbi_chrom_loc => string (3) "N/A"
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⇄ncbi_gene_id => string (3) "N/A"
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⇄ncbi_mol_weight => string (3) "N/A"
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⇄ncbi_pathways => string (3) "N/A"
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⇄sp_protein_name => string (3) "N/A"
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⇄sp_protein_name_syn => string (3) "N/A"
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⇄sp_gene_name => string (3) "N/A"
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⇄sp_entry_name => string (3) "N/A"
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⇄sp_mim => string (3) "N/A"
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⇄sp_interactions => string (3) "N/A"
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⇄products_viewed => string (1) "1"
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⇄ncbi_summary_pdns => string (3) "N/A"
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⇄products_description_extra => string (3) "N/A"
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title
Mouse Synaptosomal Protein 25kDa (SNAP-25) (a.a. 183-197) cleaved Monoclonal Antibody
datasheet
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Properties (10)
Available methods (45)
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⇄products_id => string (5) "13384"
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⇄products_model => string (8) "AAA13384"
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⇄ncbi_acc_num => string (3) "N/A"
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⇄ncbi_acc_num_related => string (3) "N/A"
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⇄ncbi_sp_acc_num_related => string (3) "N/A"
$value->a['ncbi_sp_acc_num_related']
⇄ncbi_gb_acc_num => string (3) "N/A"
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⇄sp_acc_num_syn => string (3) "N/A"
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⇄products_image => string (8) "Antibody"
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⇄sequence_positions => string (3) "N/A"
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⇄isotype => string (3) "IgG"
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$value->a['host']
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$value->a['specificity']
Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 183-197. Has been found to bind only to C. botulinum Toxin A cleaved SNAP-25 by ELISA and cell staining techniques.
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⇄storage_stability => string (71) "Upon receipt, store at -20 degree C. Avoid multiple freeze/thaw cycles."
⇄⧉app_notes => string (195) "Each laboratory should determine an optimum working titer for use in its par...
$value->a['app_notes']
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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⇄products_name_syn => string (69) "Monoclonal Antibody to Synaptosomal Protein 25 kDa (SNAP-25), cleaved"
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⇄products_gene_name => string (3) "N/A"
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⇄products_gene_name_syn => string (3) "N/A"
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⇄⧉products_description => string (96) "MAb to SNAP-25, Cleaved<br>Monoclonal Antibody to Synaptosomal Protein 25 kD...
$value->a['products_description']
MAb to SNAP-25, Cleaved<br>Monoclonal Antibody to Synaptosomal Protein 25 kDa (SNAP-25), Cleaved
⇄products_references => string (3) "N/A"
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⇄products_related_diseases => string (3) "N/A"
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⇄products_categories => string (38) "Monoclonal Antibodies for Neuroscience"
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⇄products_search_terms => string (3) "N/A"
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⇄ncbi_protein_info => string (3) "N/A"
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⇄ncbi_chrom_loc => string (3) "N/A"
$value->a['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value->a['ncbi_gene_id']
⇄ncbi_mol_weight => string (3) "N/A"
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⇄ncbi_pathways => string (3) "N/A"
$value->a['ncbi_pathways']
⇄sp_protein_name => string (3) "N/A"
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⇄sp_protein_name_syn => string (3) "N/A"
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⇄sp_gene_name => string (3) "N/A"
$value->a['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
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⇄sp_entry_name => string (3) "N/A"
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⇄sp_mim => string (3) "N/A"
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⇄sp_interactions => string (3) "N/A"
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⇄products_url => string (3) "N/A"
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⇄products_viewed => string (1) "1"
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⇄ncbi_summary_pdns => string (3) "N/A"
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⇄sp_comments_pdns => string (3) "N/A"
$value->a['sp_comments_pdns']
⇄ncbi_research_articles_pdns => string (3) "N/A"
$value->a['ncbi_research_articles_pdns']
⇄products_description_extra => string (3) "N/A"
$value->a['products_description_extra']
⇄⧉public d -> array (75)
$value->d
⇄products_id_mbs => string (6) "312597"
$value->d['products_id_mbs']
⇄products_id => string (5) "13384"
$value->d['products_id']
⇄products_quantity => string (4) "9999"
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⇄products_model => string (8) "AAA13384"
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⇄products_model_oem => string (7) "Q01235M"
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⇄products_similar => string (3) "N/A"
$value->d['products_similar']
⇄ncbi_gi_num => string (3) "N/A"
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⇄ncbi_acc_num => string (3) "N/A"
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⇄ncbi_acc_num_related => string (3) "N/A"
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⇄ncbi_sp_acc_num_related => string (3) "N/A"
$value->d['ncbi_sp_acc_num_related']
⇄ncbi_gb_acc_num => string (3) "N/A"
$value->d['ncbi_gb_acc_num']
⇄sp_acc_num => string (3) "N/A"
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⇄sp_acc_num_syn => string (3) "N/A"
$value->d['sp_acc_num_syn']
⇄products_image => string (8) "Antibody"
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⇄sequence_positions => string (3) "N/A"
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⇄sequence_length => string (3) "N/A"
$value->d['sequence_length']
⇄sequence => string (3) "N/A"
$value->d['sequence']
⇄clonality => string (10) "Monoclonal"
$value->d['clonality']
⇄isotype => string (3) "IgG"
$value->d['isotype']
⇄clone_number => string (3) "N/A"
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⇄host => string (5) "Mouse"
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⇄reactivity => string (3) "N/A"
$value->d['reactivity']
⇄⧉specificity => string (189) "Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 18...
$value->d['specificity']
Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 183-197. Has been found to bind only to C. botulinum Toxin A cleaved SNAP-25 by ELISA and cell staining techniques.
⇄purity => string (28) "Product is sterile filtered."
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⇄form => string (19) "Supernatant, Liquid"
$value->d['form']
⇄concentration => string (3) "N/A"
$value->d['concentration']
⇄storage_stability => string (71) "Upon receipt, store at -20 degree C. Avoid multiple freeze/thaw cycles."
⇄⧉app_notes => string (195) "Each laboratory should determine an optimum working titer for use in its par...
$value->d['app_notes']
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
⇄⧉specificity => string (189) "Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 18...
$value[0]['_source']['specificity']
Specific for the C.botulinum Toxin A cleaved form of SNAP-25, amino acids 183-197. Has been found to bind only to C. botulinum Toxin A cleaved SNAP-25 by ELISA and cell staining techniques.
⇄purity => string (28) "Product is sterile filtered."
$value[0]['_source']['purity']
⇄form => string (19) "Supernatant, Liquid"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (71) "Upon receipt, store at -20 degree C. Avoid multiple freeze/thaw cycles."
⇄⧉app_notes => string (195) "Each laboratory should determine an optimum working titer for use in its par...
$value[0]['_source']['app_notes']
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
aaa13384 mouse monoclonal igg product is sterile filtered supernatant liquid specific for the c.botulinum toxin a cleaved form of snap 25 amino acids 183 197 has been found to bind only c botulinum by elisa and cell staining techniques eia immunohistochemistry ihc each laboratory should determine an optimum working titer use in its particular application other applications have not tested but such assays necessarily be excluded antibody synaptosomal protein 25kda a.a mab kda antibodies neuroscience source culture immunogen synthetic peptide analogue mbs318629 cross reactivity 100 uncleaved 0 vamp2 syntaxin 1 important note centrifuge before opening ensure complete recovery vial contents snap25 acids183 reactivity100 uncleaved0 syntaxin1
⇄⧉etc_term1 => string (158) "Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue hom...
$value[1]['_source']['etc_term1']
Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Sandwich!!Detection Range||2.5-50ng/mL!!Sensitivity||0.1ng/mL
⇄⧉etc_term2 => string (408) "Intended Uses||This SNAP-25 ELISA kit is a 1.5 hour solid-phase ELISA design...
$value[1]['_source']['etc_term2']
Intended Uses||This SNAP-25 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SNAP-25. This ELISA kit for research use only, not for therapeutic applications!!!Intended Uses||This SNAP-25 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SNAP-25. This ELISA kit for research use only, not for therapeutic applications!
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⇄products_weight => string (4) "5.00"
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⇄products_status => boolean true
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⇄products_ordered => string (1) "0"
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⇄language_id => string (1) "1"
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⇄products_name => string (33) "Synaptosome Associated Protein 25"
⇄⧉products_description => string (1315) "<b>Principle of the Assay: </b>SNAP-25 ELISA kit applies the quantitative sa...
$value[1]['_source']['products_description']
<b>Principle of the Assay: </b>SNAP-25 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SNAP-25. Standards or samples are then added to the microtiter plate wells and SNAP-25 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SNAP-25 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SNAP-25 are added to each well to "sandwich" the SNAP-25 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SNAP-25 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNAP-25 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (520) "aaa16459 mouse typical testing data standard curve for reference only aaa164...
$value[1]['_source']['search_terms']
aaa16459 mouse typical testing data standard curve for reference only aaa16459_td elisa kit synaptosome associated protein 25 snap loc100533400 325296753 np_001191612.1 nm_001204683.1 neurobiology samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type sandwich detection range 2.5 50ng ml sensitivity 0.1ng intended uses this is a 1.5 hour solid phase designed the quantitative determination of research use not therapeutic applications !intended applications! protein25 range2.5 a1.5
This sterile filtered culture supernatant contains mouse monoclonal antibody 4F3-2C1. a mouse IgG<sub>2B,kappa</sub> class MAb, that is specific for the BoTox-A cleaved form of SNAP-25. This monoclonal antibody has been found to bind only to BoTox-A cleaved SNAP-25 by ELISA and cell staining techniques. Each vial contains 1.0 ml of culture supernatant.
⇄products_references => string (3) "N/A"
$value[2]['_source']['products_references']
⇄products_related_diseases => string (3) "N/A"
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⇄products_categories => string (45) "BoTox and BoTox Substrate Antibodies and Kits"
⇄⧉storage_stability => string (148) "For short term storage, store at 4 degree C.<br>For long term storage, store...
$value[3]['_source']['storage_stability']
For short term storage, store at 4 degree C.<br>For long term storage, store frozen at -20 degree C or colder.<br>Avoid repeated freeze/thaw cycles.
⇄app_tested => string (3) "N/A"
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⇄⧉app_notes => string (301) "Immunocytochemical Staining of Rat Brain Section: This monoclonal antibody c...
$value[3]['_source']['app_notes']
Immunocytochemical Staining of Rat Brain Section: This monoclonal antibody containing culture supernatant has been found to stain fixed normal rat brain sections in cells known to express SNAP-25 at a dilution of 1:100. The immunocytochemical staining was observed in puncta and at synaptic terminals.
This sterile filtered culture supernatant contains mouse monoclonal antibody 5G4-1D1. a mouse IgG class MAb. that is specific for both the intact whole form of SNAP-25 and the BoTox-A cleaved form of SNAP-25. This monoclonal antibody has been found to bind to cleaved and uncleaved SNAP-25 by ELISA and cell staining techniques. Each vial contains 1.0 ml of culture supernatant.
⇄products_references => string (3) "N/A"
$value[3]['_source']['products_references']
⇄⧉products_related_diseases => string (228) "Mental Disorders||97!!Attention Deficit Disorder with Hyperactivity||43!!Sch...
⇄⧉ncbi_protein_info => string (188) "synaptosomal-associated protein 25; SUP; super protein; OTTHUMP00000030267; ...
$value[3]['_source']['ncbi_protein_info']
synaptosomal-associated protein 25; SUP; super protein; OTTHUMP00000030267; OTTHUMP00000030268; synaptosomal-associated 25 kDa protein; resistance to inhibitors of cholinesterase 4 homolog
⇄⧉products_description => string (704) "Intended Uses: This SNAP-tag ELISA kit is intended Laboratory for Research u...
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Intended Uses: This SNAP-tag ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SNAP-tag in the sample, this SNAP-tag ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SNAP-tag concentration. The concentration of SNAP-tag in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄products_references => string (3) "N/A"
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⇄products_related_diseases => string (3) "N/A"
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⇄⧉search_terms => string (187) "aaa23666 human typical testing data standard curve for reference only aaa236...
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aaa23666 human typical testing data standard curve for reference only aaa23666_sc elisa kit snap tag sensitivity 1 pg ml intra assay precision cv is less than 15 inter sensitivity1 than15
Human<br>Predicted to React with: Bovine, Chick, Pongo abelii (Species predicted to react based on 100% sequence homology)
⇄specificity => string (13) "Human SNAP-25"
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⇄purity => string (28) "Protein A & Antigen Affinity"
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⇄form => string (38) "Liquid; 0.2um filtered solution in PBS"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (261) "This antibody can be stored at 2-8 degree C for one month without detectable...
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This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
⇄app_tested => string (56) "Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)"
⇄⧉testing_protocols => string (1568) "IHC (Immunohistochemistry)||Immunochemical staining of human SNAP25 in rat l...
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IHC (Immunohistochemistry)||Immunochemical staining of human SNAP25 in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.||AAA27737_IHC7.png!!IHC (Immunohistchemistry)||Immunochemical staining of human SNAP25 in rat brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.||AAA27737_IHC6.png!!IHC (Immunohistochemistry)||Immunochemical staining of human SNAP25 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.||AAA27737_IHC5.png!!IHC (Immunohistochemistry)||Immunochemical staining of human SNAP25 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.||AAA27737_IHC4.png!!IHC (Immunohistochemistry)||Immunochemical staining of human SNAP25 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.||AAA27737_IHC3.png!!IHC (Immunohistochemistry)||Immunochemical staining of human SNAP25 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.||AAA27737_IHC2.png!!WB (Western Blot)||Anti-SNAP25 rabbit polyclonal antibody at 1:500 dilution<br> Lane A: SH-SY5Y Whole Cell Lysate<br> Lysates/proteins at 30 ug per lane.<br> Secondary<br> Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.<br> Developed using the ECL technique.<br> Performed under reducing conditions.<br> Predicted band size:23 kDa<br> Observed band size:26 kDa<br>||AAA27737_WB.png
⇄⧉etc_term1 => string (110) "Immunogen||A synthetic peptide corresponding to the N-terminus of the Human ...
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Immunogen||A synthetic peptide corresponding to the N-terminus of the Human SNAP-25!!Conjugation||Unconjugated
⇄⧉etc_term2 => string (170) "Preparation||Produced in rabbits immunized with a synthetic peptide correspo...
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Preparation||Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human SNAP-25, and purified by antigen affinity chromatography.
⇄⧉products_description => string (1446) "Synaptosomal-associated protein 25, also known as Super protein, Synaptosoma...
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Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein that belongs to the SNAP-25 family. SNAP25/SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25/SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25/SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25/SUP is a Q-SNARE protein contributing two alpha-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25/SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25/SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25/SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25/SUP inhibits P/Q-and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25/SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
⇄⧉products_references => string (284) "Hodel A, et al., 1998, Int. J. Biochem. Cell Biol. 30 (10): 1069-73. Sudhof ...
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Hodel A, et al., 1998, Int. J. Biochem. Cell Biol. 30 (10): 1069-73. Sudhof TC, et al., 2002, Nat Rev Neurosci 3 (8): 641-653. Chapman ER et al., 2002, Nat. Rev. Mol. Cell Biol. 3(7): 498-508. Chen X., et al., 2002, Neuron 33:397-409. Huang Q., et al., 2008, FEBS Lett. 582:1431-1436.
⇄⧉products_related_diseases => string (228) "Nervous System Diseases||114!!Drug Toxicity||36!!Paralysis||21!!Cognition Di...
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Nervous System Diseases||114!!Drug Toxicity||36!!Paralysis||21!!Cognition Disorders||15!!Memory Disorders||14!!Movement Disorders||12!!Learning Disorders||12!!Neurobehavioral Manifestations||12!!Seizures||11!!Bipolar Disorder||9
⇄products_categories => string (3) "N/A"
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⇄ncbi_full_name => string (50) "synaptosomal-associated protein 25 isoform SNAP25A"
⇄⧉search_terms => string (1133) "aaa27737 rabbit human predicted to react with bovine chick pongo abelii spec...
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aaa27737 rabbit human predicted to react with bovine chick pongo abelii species based on 100 sequence homology polyclonal igg protein a antigen affinity liquid 0.2um filtered solution in pbs snap 25 western blot wb immunohistochemistry paraffin ihc p 1:500 1:2000 anti snap25 antibody at dilution lane sh sy5y whole cell lysate lysates proteins 30 ug per secondary goat h+l hrp 1 10000 developed using the ecl technique performed under reducing conditions band size:23 kda observed size:26 aaa27737_wb immunochemical staining of brain 1:1000 formalin fixed embedded sections aaa27737_ihc2 liver aaa27737_ihc3 mouse aaa27737_ihc4 aaa27737_ihc5 rat aaa27737_ihc6 aaa27737_ihc7 pab purified synaptosomal associated 25kda ba416n4.2 dj1068f16.2 ric 4 ric4 sec9 isoform snap25a sup super resistance inhibitors cholinesterase homolog 23,315 da snp25_human 18765733 np_003072.2 p60880 nm_003081.3 p13795 p36974 p70557 p70558 q53em2 q5u0b5 q8ixk3 q96fm2 b2rau4 d3dw16 d3dw17 600322 immunogen synthetic peptide corresponding n terminus conjugation unconjugated preparation produced rabbits immunized and by chromatography on100 proteins30 hrp1
⇄⧉testing_protocols => string (1520) "FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with Glucose 6 ...
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FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with Glucose 6 Phosphate Dehydrogenase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).||AAA30344_FCM7.jpg!!ICC (Immunocytochemistry)||ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30344_ICC6.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.||AAA30344_IHC5.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.||AAA30344_IHC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.||AAA30344_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.||AAA30344_IHC2.jpg!!WB (Western Blot)||Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1, 000 dilution.||AAA30344_WB.jpg
⇄⧉products_description => string (764) "Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pe...
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Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pentose phosphate pathway. It is a member of the glucose-6-phosphate dehydrogenase family of proteins. G6PD is a ubiquitous enzyme that produces pentose sugars for nucleic acid synthesis, but is also involved in carbohydrate degradation, as it is one of the main producers of NADPH reducing power. G6PD has NADP as a co-factor and structural element. It can be found as a homodimer or homotetramer, and is primarily detected in lymphoblasts, granulocytes and sperm. Defects in G6PD can cause chronic non-spherocytic hemolytic anemia (CNSHA), especially in areas in which malaria is an epidemic. Individuals with a high level of G6PD-deficiency are at higher risk of acute hemolytic attacks.
aaa30344 rabbit human monoclonal ja31 40 proa affinity purified 1*tbs ph7.4 1 bsa glycerol preservative 0.05 sodium azide western blot wb immunocytochemistry icc immunohistochemistry ihc flow cytometry fc facs 1:500 1:2000 1:50 1:200 1:100 analysis of glucose 6 phosphate dehydrogenase on a549 cell lysate using anti antibody at 000 dilution aaa30344_wb immunohistochemical paraffin embedded mouse testes tissue counter stained with hematoxylin aaa30344_ihc2 liver aaa30344_ihc3 stomach cancer aaa30344_ihc4 skeletal muscle aaa30344_ihc5 staining in mcf 7 cells green the nuclear stain is dapi blue were fixed paraformaldehyde permeabilised 0.25 triton x100 pbs aaa30344_icc6 cytometric hela 100 red compared an unlabelled control without incubation primary black aaa30344_fc7 g6pd g6pd_human g6pd1 g6pdx met19 pos10 zwf1p 59,257 da 1203978 aaa92653.1 p11413 q16000 q16765 q8iu70 q8iu88 q8iua6 q96pq2 d3dwx9 305900 total protein ab type recombinant immunogen conjugation unconjugated ja3140 ph7.41 glucose6 at000 mcf7 hela100
⇄⧉products_description => string (894) "Intended Uses: This sandwich kit is for the accurate quantitative detection ...
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Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Synaptosomal-Associated Protein 25 (also known as SNAP25) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.<br><br>Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SNAP25 antibody. SNAP25 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SNAP25 Antibody is added and binds to SNAP25 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SNAP25 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SNAP25. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (221) "Nervous System Diseases||117!!Paralysis||21!!Cognition Disorders||17!!Pain||...
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Nervous System Diseases||117!!Paralysis||21!!Cognition Disorders||17!!Pain||15!!Memory Disorders||15!!Movement Disorders||13!!Seizures||12!!Neurobehavioral Manifestations||12!!Learning Disorders||12!!Diabetes Mellitus||10
⇄products_categories => string (3) "N/A"
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⇄ncbi_full_name => string (44) "synaptosomal-associated protein 25 isoform a"
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⇄ncbi_full_name_syn => string (33) "synaptosome associated protein 25"
⇄⧉search_terms => string (477) "aaa11353 human typical testing data standard curve for reference only aaa113...
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aaa11353 human typical testing data standard curve for reference only aaa11353_sc elisa kit synaptosomal associated protein 25 snap25 isoform a synaptosome snap 25b 25a 23,336 da super sup kda 396080316 np_001257505.1 p60881 nm_001270576.1 p13795 p36974 p70557 p70558 q8ixk3 q96fm2 q9br45 samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 0.05ng ml 20ng sensitivity 0.023ng intra cv<8 inter cv<10 protein25
⇄⧉testing_protocols => string (1603) "FCM (Flow Cytometry)||Flow cytometric analysis of A549 cells with Glucose 6 ...
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FCM (Flow Cytometry)||Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.||AAA30477_FCM7.jpg!!ICC (Immunocytochemistry)||ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30477_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30477_ICC5.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.||AAA30477_IHC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.||AAA30477_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.||AAA30477_IHC2.jpg!!WB (Western Blot)||Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1, 000 dilution.||AAA30477_WB.jpg
⇄⧉etc_term1 => string (131) "Antibody Type||Recombinant Antibody!!Immunogen||Recombinant protein correspo...
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Antibody Type||Recombinant Antibody!!Immunogen||Recombinant protein corresponding to human Glucose 6 phosphate isomerase 300-500aa.
⇄⧉products_description => string (473) "Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphoh...
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Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment.
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (207) "Nervous System Diseases||1479!!Anemia||528!!Death||489!!Inflammation||457!!D...
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Nervous System Diseases||1479!!Anemia||528!!Death||489!!Inflammation||457!!Disease Models, Animal||453!!Anemia, Hemolytic||449!!Necrosis||433!!Adenocarcinoma||380!!Ovarian Neoplasms||333!!Heart Diseases||247
⇄products_categories => string (16) "Total protein Ab"
⇄reactivity => string (31) "Rat, Hamster Gerbil and Porcine"
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⇄⧉specificity => string (161) "Recognizes human SNAP-25; an antigen of 26-27kD molecular weight. Will react...
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Recognizes human SNAP-25; an antigen of 26-27kD molecular weight. Will react with SNAP-25 from COS cells, but not with the fusion protein from bacterial systems.
⇄purity => string (77) "Purified by Protein A affinity chromatography from tissue culture supernatent"
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⇄form => string (56) "Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide."
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⇄concentration => string (6) "1mg/mL"
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⇄⧉storage_stability => string (272) "May be stored at 4 degree for short term only. Aliquot to avoid repeated fre...
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May be stored at 4 degree for short term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree. Aliquots are stable for 12 months after receipt. For maximum recovery of profuct, centrifuge the original vial after thawing and prior to removing the cap.
⇄app_tested => string (58) "ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)"
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⇄⧉app_notes => string (457) "Immunohistochemistry (Paraffin): 1:2000-1:5000<br>Does not require protein d...
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Immunohistochemistry (Paraffin): 1:2000-1:5000<br>Does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.<br>Western Blot: 1:500-1:5000<br><br>Optimal working dilutions to be determined by researcher.<br><br><br><br><br>Recommended Secondary Antibodies:<br><br>IgG, H&L (AP) Pab Gt xMo<br>IgG, H&L (FITC) Pab Gt xMo<br>IgG, H&L (HRP) Pab Gt xMo<br>IgG,(HRP) Pab Rb xMo<br><br>IgG (PE) Pab Gt xMo
⇄⧉testing_protocols => string (102) "IHC (Immunohistochemistry)||IHC staining of human cerebellar neurons using A...
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IHC (Immunohistochemistry)||IHC staining of human cerebellar neurons using AAA14700.||AAA14700_IHC.jpg
⇄etc_term1 => string (50) "Immunogen||Crude human synaptic immunoprecipitate."
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⇄⧉etc_term2 => string (120) "Hybridoma||NSO myeloma cells with spleen cells from Balb/c mice.!!IHC Positi...
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Hybridoma||NSO myeloma cells with spleen cells from Balb/c mice.!!IHC Positive Control||Brain!!Grade||Afffinity Purified
⇄⧉products_description => string (177) "Research studies have used this antibody to study the distribution of synapt...
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Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy (1, 3, 8).
⇄products_references => string (3) "N/A"
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⇄products_categories => string (31) "Antibodies; Abs to Neuroscience"
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⇄ncbi_full_name => string (3) "N/A"
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⇄ncbi_pathways => string (3) "N/A"
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⇄sp_entry_name => string (3) "N/A"
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⇄products_url => string (3) "N/A"
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⇄⧉search_terms => string (784) "aaa14700 mouse monoclonal igg1 3h2886 sp12 affinity purified by protein a ch...
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aaa14700 mouse monoclonal igg1 3h2886 sp12 affinity purified by protein a chromatography supplied as liquid in pbs ph 7.2 0.09 sodium azide recognizes human snap 25 an antigen of 26 27kd molecular weight will react with from cos cells but not the fusion bacterial systems species crossreactivity rat hamster gerbil and porcine elisa eia western blot wb immunohistochemistry ihc suitable for use dilution paraffin 1:2000 1:5000 does require digestion or retrieval using heat treatment prior to staining sections 1:500 optimal working dilutions be determined researcher cerebellar neurons aaa14700_ihc antibody synaptosomal associated anti np_001266 p60880 antibodies abs neuroscience immunogen crude synaptic immunoprecipitate hybridoma nso myeloma spleen balb c mice ph7.2 snap25 of26
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of P...
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This assay has high sensitivity and excellent specificity for detection of PROS. No significant cross-reactivity or interference between PROS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PROS and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (34) "Store all reagents at 2-8 degree C"
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄etc_term2 => string (3) "N/A"
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⇄products_weight => string (4) "5.00"
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⇄manufacturers_id => string (3) "720"
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⇄language_id => string (1) "1"
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⇄products_name => string (9) "Protein S"
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⇄products_name_oem => string (25) "Mouse Protein S ELISA Kit"
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⇄⧉products_name_syn => string (94) "Mouse Protein S (Protein S) ELISA Kit; Mouse Protein S ELISA Kit; Protein S;...
$value[10]['_source']['products_name_syn']
Mouse Protein S (Protein S) ELISA Kit; Mouse Protein S ELISA Kit; Protein S; Protein S (Mouse)
⇄products_gene_name => string (9) "Protein S"
$value[10]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[10]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1503) "Principle of the Assay: PROS ELISA kit applies the quantitative sandwich enz...
$value[10]['_source']['products_description']
Principle of the Assay: PROS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PROS. Standards or samples are then added to the microtiter plate wells and PROS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PROS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PROS are added to each well to "sandwich" the PROS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PROS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROS concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This PROS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PROS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (506) "aaa16417 mouse this assay has high sensitivity and excellent specificity for...
$value[10]['_source']['search_terms']
aaa16417 mouse this assay has high sensitivity and excellent specificity for detection of pros no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16417_sc elisa kit protein s 7717469 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative sandwich 1.0pg ml
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of G...
$value[11]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of GLU. No significant cross-reactivity or interference between GLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLU and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[11]['_source']['purity']
⇄form => string (3) "N/A"
$value[11]['_source']['form']
⇄concentration => string (3) "N/A"
$value[11]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1374) "Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoa...
$value[11]['_source']['products_description']
Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLU antibody and an GLU-HRP conjugate. The assay sample and buffer are incubated together with GLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLU concentration since GLU from samples and GLU-HRP conjugate compete for the anti-GLU antibody binding site. Since the number of sites is limited, as more sites are occupied by GLU from the sample, fewer sites are left to bind GLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLU concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This GLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (513) "aaa27242 rat this assay has high sensitivity and excellent specificity for d...
$value[11]['_source']['search_terms']
aaa27242 rat this assay has high sensitivity and excellent specificity for detection of glu no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27242_sc elisa kit glucose samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 mmol l competitive0.1
⇄⧉testing_protocols => string (2232) "FCM (Flow Cytometry)||Flow cytometric analysis of SH-SY-5Y cells with Fructo...
$value[12]['_source']['testing_protocols']
FCM (Flow Cytometry)||Flow cytometric analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.||AAA30431_FCM10.jpg!!ICC (Immunocytochemistry)||ICC staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30431_ICC9.jpg!!ICC (Immunocytochemistry)||ICC staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30431_ICC8.jpg!!ICC (Immunocytochemistry)||ICC staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30431_ICC7.jpg!!IHC (Immunohistchemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.||AAA30431_IHC6.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.||AAA30431_IHC5.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.||AAA30431_IHC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.||AAA30431_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.||AAA30431_IHC2.jpg!!WB (Western Blot)||Western blot analysis of Fructose 6 Phosphate Kinase on PC-3M cell lysate using anti-Fructose 6 Phosphate Kinase antibody at 1/500 dilution.||AAA30431_WB.jpg
6 Phosphofructokinase Muscle Type antibody; 6-phosphofructokinase antibody; 6-phosphofructokinase muscle type antibody; EC 2.7.1.1 antibody; EC 2.7.1.11 antibody; GSD7 antibody; K6PF_HUMAN antibody; MGC8699 antibody; muscle type antibody; PFK; muscle type antibody; PFK-A antibody; PFKA antibody; PFKL antibody; PFKM antibody; PFKP antibody; PFKX antibody; Phosphofructo 1 Kinase Isozyme A antibody; Phosphofructo-1-kinase isozyme A antibody; Phosphofructokinase 1 antibody; Phosphofructokinase M antibody; Phosphofructokinase; muscle antibody; Phosphofructokinase; muscle type antibody; Phosphofructokinase; polypeptide X antibody; Phosphofructokinase-M antibody; Phosphohexokinase antibody
⇄products_gene_name => string (3) "N/A"
$value[12]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[12]['_source']['products_gene_name_syn']
⇄⧉products_description => string (250) "Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1, 6-bis...
$value[12]['_source']['products_description']
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1, 6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.
⇄products_references => string (3) "N/A"
$value[12]['_source']['products_references']
⇄⧉products_related_diseases => string (261) "Nervous System Diseases||6!!Glycogen Storage Disease Type VII||6!!Liver Dise...
⇄⧉ncbi_pathways => string (442) "Biosynthesis Of Amino Acids Pathway||790012!!Biosynthesis Of Amino Acids Pat...
$value[12]['_source']['ncbi_pathways']
Biosynthesis Of Amino Acids Pathway||790012!!Biosynthesis Of Amino Acids Pathway||795174!!Carbon Metabolism Pathway||814926!!Carbon Metabolism Pathway||817567!!Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway||835393!!Fructose And Mannose Metabolism Pathway||82930!!Fructose And Mannose Metabolism Pathway||291!!Galactose Metabolism Pathway||82931!!Galactose Metabolism Pathway||292!!Glucose Metabolism Pathway||106199
⇄⧉search_terms => string (1305) "aaa30431 rabbit human mouse rat monoclonal ju53 31 proa affinity purified 1*...
$value[12]['_source']['search_terms']
aaa30431 rabbit human mouse rat monoclonal ju53 31 proa affinity purified 1*tbs ph7.4 1 bsa 40 glycerol preservative 0.05 sodium azide western blot wb immunocytochemistry icc immunofluorescence if immunohistochemistry ihc flow cytometry fc facs 1:500 1:1000 1:50 1:200 1:100 analysis of fructose 6 phosphate kinase on pc 3m cell lysate using anti antibody at 500 dilution aaa30431_wb immunohistochemical paraffin embedded brain tissue counter stained with hematoxylin aaa30431_ihc2 liver cancer aaa30431_ihc3 skeletal muscle aaa30431_ihc4 thyroid gland aaa30431_ihc5 kidney aaa30431_ihc6 staining in lovo cells green the nuclear stain is dapi blue were fixed paraformaldehyde permeabilised 0.25 triton x100 pbs aaa30431_icc7 aaa30431_icc8 sh sy 5y aaa30431_icc9 cytometric 100 red compared an unlabelled control without incubation primary black alexa fluor 488 conjugated goat igg was used as secondary aaa30431_fc10 phosphofructokinase type ec 2.7.1.1 2.7.1.11 gsd7 k6pf_human mgc8699 pfk a pfka pfkl pfkm pfkp pfkx phosphofructo isozyme m polypeptide x phosphohexokinase isoform 2 pfk1 780 4505749 np_000280.1 p08237 nm_000289.5 q16814 q16815 q6ztt1 j3knx3 232800 total protein ab recombinant immunogen conjugation unconjugated ju5331 ph7.41 bsa40 fructose6 at500 cytometric100 fluor488 isoform2 pfk1780
⇄⧉storage_stability => string (269) "Upon receipt, store the Glucose Standard, Colorimetric Probe, HRP, and Gluco...
$value[13]['_source']['storage_stability']
Upon receipt, store the Glucose Standard, Colorimetric Probe, HRP, and Glucose Oxidase at -20 degree C<br> The Fluorescence Probe is light sensitive and must be stored accordingly<br> Avoid multiple freeze/thaw cycles<br> Store the 10X Assay Buffer at room temperature.
⇄⧉products_description => string (2124) "Principle of the Assay: Total Glucose Assay Kit measures total glucose withi...
$value[13]['_source']['products_description']
Principle of the Assay: Total Glucose Assay Kit measures total glucose within food or biological samples. Glucose is oxidized by glucose oxidase into D-gluconic acid plus hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific colorimetric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of glucose standard within the 96-well microtiter plate format. Samples and standards are incubated for 30-45 minutes and then read with a standard 96-well colorimetric plate reader (Figure 1).<br><br>Background/Introduction: Glucose is a sugar used as an important source of energy in plants, prokaryotes and eukaryotes via processes such as respiration and fermentation. In plants, algae, and cyanobacteria, the energy of light is synthesized into the storage form of sugars such as glucose. More specifically, in a downstream process known as the Calvin cycle, carbon dioxide is incorporated into organic carbon compounds, like ribulose bisphosphate. Using ATP and NADPH from upstream light-dependent reactions, the resulting compounds are then reduced and removed to form further carbohydrates, such as glucose. In animals, through the process of glycolysis followed by the citric acid cycle, glucose is broken down to water and CO2, resulting in energy from ATP formation. Glucose is often stored as a polymer such as glycogen. In humans, glucose is commonly measured in blood samples. Bloodstream levels of glucose are normally under tight regulation; however, high levels measured in fasting individuals may indicate prediabetes or diabetes. Glucose Assay Kit is a simple colorimetric assay that measures the amount of total glucose present in foods or biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 500 assays, including blanks, glucose standards and unknown samples. Sample glucose concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 6.25 uM glucose.
⇄⧉products_description => string (527) "Background: Glucose (C6H12O6) is a key diagnostic parameter for many metabol...
$value[14]['_source']['products_description']
Background: Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. The assay is initiated with the enzymatic catalysis of glucose by glucose oxidase. The enzyme catalysed reaction products H2O2 react with the substrate, and can be measured at a colorimetric readout at 505 nm.
⇄⧉search_terms => string (251) "aaa27844 general functional assay typical testing data standard curve for re...
$value[14]['_source']['search_terms']
aaa27844 general functional assay typical testing data standard curve for reference only aaa27844_sc kit glucose tissue microplate samples serum plasma extracts cell lysate culture and other biological fluids detection range 0.1 mmol l 10 range0.1 l10
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of G...
$value[15]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of GLU. No significant cross-reactivity or interference between GLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLU and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[15]['_source']['purity']
⇄form => string (3) "N/A"
$value[15]['_source']['form']
⇄concentration => string (3) "N/A"
$value[15]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1377) "Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoa...
$value[15]['_source']['products_description']
Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLU antibody and an GLU-HRP conjugate. The assay sample and buffer are incubated together with GLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLU concentration since GLU from samples and GLU-HRP conjugate compete for the anti-GLU antibody binding site. Since the number of sites is limited, as more sites are occupied by GLU from the sample, fewer sites are left to bind GLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLU concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This GLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (515) "aaa27182 bovine this assay has high sensitivity and excellent specificity fo...
$value[15]['_source']['search_terms']
aaa27182 bovine this assay has high sensitivity and excellent specificity for detection of glu no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27182_sc elisa kit glucose samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 mg ml competitive0.1
⇄⧉storage_stability => string (269) "Upon receipt, store the Glucose Standard, Colorimetric Probe, HRP, and Gluco...
$value[16]['_source']['storage_stability']
Upon receipt, store the Glucose Standard, Colorimetric Probe, HRP, and Glucose Oxidase at -20 degree C<br> The Fluorescence Probe is light sensitive and must be stored accordingly<br> Avoid multiple freeze/thaw cycles<br> Store the 10X Assay Buffer at room temperature.
⇄⧉products_description => string (1044) "Background: Glucose is a sugar used as an important source of energy in plan...
$value[16]['_source']['products_description']
Background: Glucose is a sugar used as an important source of energy in plants, prokaryotes and eukaryotes via processes such as respiration and fermentation. In plants, algae, and cyanobacteria, the energy of light is synthesized into the storage form of sugars such as glucose. More specifically, in a downstream process known as the Calvin cycle, carbon dioxide is incorporated into organic carbon compounds, like ribulose bisphosphate. Using ATP and NADPH from upstream light-dependent reactions, the resulting compounds are then reduced and removed to form further carbohydrates, such as glucose. In animals, through the process of glycolysis followed by the citric acid cycle, glucose is broken down to water and CO2, resulting in energy from ATP formation. Glucose is often stored as a polymer such as glycogen. In humans, glucose is commonly measured in blood samples. Bloodstream levels of glucose are normally under tight regulation (Table 1); however, high levels measured in fasting individuals may indicate prediabetes or diabetes.
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of G...
$value[17]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of GLU. No significant cross-reactivity or interference between GLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLU and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[17]['_source']['purity']
⇄form => string (3) "N/A"
$value[17]['_source']['form']
⇄concentration => string (3) "N/A"
$value[17]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1376) "Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoa...
$value[17]['_source']['products_description']
Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLU antibody and an GLU-HRP conjugate. The assay sample and buffer are incubated together with GLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLU concentration since GLU from samples and GLU-HRP conjugate compete for the anti-GLU antibody binding site. Since the number of sites is limited, as more sites are occupied by GLU from the sample, fewer sites are left to bind GLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLU concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This GLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (534) "aaa27068 mouse this assay has high sensitivity and excellent specificity for...
$value[17]['_source']['search_terms']
aaa27068 mouse this assay has high sensitivity and excellent specificity for detection of glu no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27068_sc elisa kit glucose signal transduction samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 mg ml competitive0.1
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of G...
$value[18]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of GLU. No significant cross-reactivity or interference between GLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLU and all the analogues, therefore, cross reaction may still exist in some cases.
⇄⧉products_description => string (1376) "Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoa...
$value[18]['_source']['products_description']
Principle of the Assay: GLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLU antibody and an GLU-HRP conjugate. The assay sample and buffer are incubated together with GLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLU concentration since GLU from samples and GLU-HRP conjugate compete for the anti-GLU antibody binding site. Since the number of sites is limited, as more sites are occupied by GLU from the sample, fewer sites are left to bind GLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLU concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This GLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (536) "aaa27388 human this assay has high sensitivity and excellent specificity for...
$value[18]['_source']['search_terms']
aaa27388 human this assay has high sensitivity and excellent specificity for detection of glu no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27388_sc elisa kit glucose 1100620057 apb58760.1 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 mg ml competitive0.1
⇄⧉specificity => string (184) "This assay has high sensitivity and excellent specificity for detection of P...
$value[19]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of Pig 25-HVD3. No significant cross-reactivity or interference between Pig 25- HVD3 and analogues was observed.
⇄purity => string (3) "N/A"
$value[19]['_source']['purity']
⇄form => string (3) "N/A"
$value[19]['_source']['form']
⇄concentration => string (3) "N/A"
$value[19]['_source']['concentration']
⇄storage_stability => string (22) "Store at 2-8 degree C."
$value[19]['_source']['storage_stability']
⇄app_tested => string (3) "N/A"
$value[19]['_source']['app_tested']
⇄app_notes => string (3) "N/A"
$value[19]['_source']['app_notes']
⇄testing_protocols => string (3) "N/A"
$value[19]['_source']['testing_protocols']
⇄⧉etc_term1 => string (182) "Assay Type||Quantitative Sandwich!!Samples||Serum, plasma and other biologic...
$value[19]['_source']['etc_term1']
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma and other biological fluids!!Detection Range||3.12 ng/mL - 200 ng/mL!!Sensitivity||1.04 ng/mL (mean of 6 independent assays)
⇄⧉etc_term2 => string (420) "Intra-assay Precision||Intra-assay Precision (Precision within an assay) Thr...
$value[19]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄⧉products_description => string (922) "Principle of the Assay: This assay employs a two-site sandwich ELISA to quan...
$value[19]['_source']['products_description']
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative 25-HVD3 in Pig serum, plasma. An antibody specific for 25-HVD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 25-HVD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for 25-HVD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 25-HVD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.<br><br>Intended Uses: For the quantitative detection of Pig 25-hydroxy vitamin D3 (25-HVD3) concentration in serum, plasma and other biological fluids.
⇄⧉search_terms => string (501) "aaa13285 pig this assay has high sensitivity and excellent specificity for d...
$value[19]['_source']['search_terms']
aaa13285 pig this assay has high sensitivity and excellent specificity for detection of mouse wt1 no significant cross reactivity or interference between analogues was observed elisa kit 25 hydroxy vitamin d3 hvd3 samples serum plasma other biological fluids type quantitative sandwich range 15.6 pg ml 1000 7.8 mean 6 independent assays intra precision within an three known concentration were tested twenty times on one plate to assess cv<8 inter in forty separate cv = sd meanx100 cv<12 kit25 mean6
