Canine Anti-acrosin antibodies ELISA Kit | ACR elisa kit

Canine Anti-acrosin antibodies ELISA Kit

Cat. Num. AAA734087

• Reactivity: Canine
• Synonyms: Anti-acrosin antibodies; Canine Anti-acrosin antibodies ELISA Kit; ACR elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ACRAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ACRAb. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACR elisa kit

Principle of the Assay: ACRAb ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with acrosin antigen. Standards or samples are then added to the microtiter plate wells and ACRAb if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of ACRAb present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated acrosin antigen is added to each well to "sandwich" the ACRAb immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACRAb and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACRAb concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ACR elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 47678263
• NCBI GeneID: 49
• UniProt Accession #: P10323; Q6ICK2
• Molecular Weight: 45,847 Da
• NCBI Official Full Name: ACR
• NCBI Official Synonym Full Names: acrosin
• NCBI Official Symbol: ACR
• NCBI Protein Information: acrosin; proacrosin; preproacrosin; acrosin light and heavy chain prepropeptide
• UniProt Protein Name: Acrosin
• Protein Family: Acrosin
• UniProt Gene Name: ACR
• UniProt Synonym Gene Names: ACRS
• UniProt Entry Name: ACRO_HUMAN

NCBI Description

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Uniprot Description

ACR: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.10; Protease

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: protein complex; extracellular region; Golgi-associated vesicle; nucleus; acrosomal matrix

Molecular Function: mannose binding; protein binding; copper ion binding; DNA binding; amidase activity; zinc ion binding; serine-type endopeptidase activity; drug binding; fucose binding

Biological Process: response to steroid hormone stimulus; binding of sperm to zona pellucida; single fertilization; adenylate cyclase activation; acrosome reaction; penetration of zona pellucida; multicellular organism reproduction; acrosome matrix dispersal