Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit COMMD5 Polyclonal Antibody | anti-COMMD5 antibody
COMMD5 Antibody
Gene Names
COMMD5; HCARG; HT002
Reactivity
Human, Mouse, Rat, MonkeyPredicted: Pig, Bovine, Horse, Sheep
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
COMMD5; Polyclonal Antibody; COMMD5 Antibody; anti-COMMD5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Predicted: Pig, Bovine, Horse, Sheep
Predicted: Pig, Bovine, Horse, Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
COMMD5 Antibody detects endogenous levels of COMMD5.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
224
Applicable Applications for anti-COMMD5 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human COMMD5.
Tissue Specificity
Highly expressed in heart, stomach, jejunum, kidney, liver, and adrenal gland. Expression was generally higher in adult organs than in fetal tissues, particularly in heart, kidney, and liver.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from VERO, using COMMD5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from VERO, using COMMD5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells, using COMMD5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells, using COMMD5 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-COMMD5 antibody
Description: May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (PubMed:21778237). Negatively regulates cell proliferation. Negatively regulates cell cycle G2/M phase transition probably by transactivating p21/CDKN1A through the p53/TP53-independent signaling pathway. Involved in kidney proximal tubule morphogenesis (By similarity). Down-regulates activation of NF-kappa-B (PubMed:15799966).
Subunit Structure: Interacts (via COMM domain) with COMMD1 (via COMM domain). Interacts with RELA, RELB, NFKB1/p105. Interacts with CCDC22, CCDC93, SCNN1B, CUL2, CUL3, CUL4A, CUL4B, CUL7.
Subunit Structure: Interacts (via COMM domain) with COMMD1 (via COMM domain). Interacts with RELA, RELB, NFKB1/p105. Interacts with CCDC22, CCDC93, SCNN1B, CUL2, CUL3, CUL4A, CUL4B, CUL7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 25 kDa
Predicted: 25 kDa
Predicted: 25 kDa
NCBI Official Full Name
COMM domain-containing protein 5
NCBI Official Synonym Full Names
COMM domain containing 5
NCBI Official Symbol
COMMD5
NCBI Official Synonym Symbols
HCARG; HT002
NCBI Protein Information
COMM domain-containing protein 5
UniProt Protein Name
COMM domain-containing protein 5
Protein Family
UniProt Gene Name
COMMD5
UniProt Synonym Gene Names
HT002; HCaRG
UniProt Entry Name
COMD5_HUMAN
Research Articles on COMMD5
Similar Products
Product Notes
The COMMD5 commd5 (Catalog #AAA9611336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COMMD5 Antibody reacts with Human, Mouse, Rat, Monkey Predicted: Pig, Bovine, Horse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's COMMD5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the COMMD5 commd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COMMD5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
