Rabbit Collagen XVII/COL17A1 Polyclonal Antibody | anti-COL17A1 antibody
Anti-Collagen XVII/COL17A1 Antibody
Gene Names
COL17A1; ERED; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
Collagen XVII/COL17A1; Polyclonal Antibody; Anti-Collagen XVII/COL17A1 Antibody; Collagen alpha-1 (XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; 120 kDa linear IgA dermatosis antigen; Linear IgA disease antigen 1; LAD-1; 97 kDa linear IgA bullous dermatosis antigen; 97 kDa LAD antigen; 97-LAD; Linear IgA bullous disease antigen of 97 kDa; LABD97; COL17A1; BP180; BPAG2; Collagen type XVII alpha 1 chain; anti-COL17A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
1,415
Applicable Applications for anti-COL17A1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
IHC-P: 0.5-1 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Immunogen
A synthetic peptide corresponding to a sequence of human Collagen XVII/COL17A1 (KVFSAYSNVTADLMDFFQTYGAIQ).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
Related Product Information for anti-COL17A1 antibody
Description: Rabbit IgG polyclonal antibody for Collagen XVII/COL17A1 detection. Tested with WB, IHC-P in Human; Mouse; Rat.
Background: Collagen XVII, previously called BP180, is a transmembrane protein which plays a critical role in maintaining the linkage between the intracellular and the extracellular structural elements involved in epidermal adhesion. It is mapped to 10q25.1. This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form.
Background: Collagen XVII, previously called BP180, is a transmembrane protein which plays a critical role in maintaining the linkage between the intracellular and the extracellular structural elements involved in epidermal adhesion. It is mapped to 10q25.1. This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form.
References
1. Chavanas, S., Gache, Y., Tadini, G., Pulkkinen, L., Uitto, J., Ortonne, J. P., Meneguzzi, G. A homozygous in-frame deletion in the collagenous domain of bullous pemphigoid antigen BP180 (type XVII collagen) causes generalized atrophic benign epidermolysis bullosa. J. Invest. Derm. 109: 74-78, 1997. Note: Erratum: J. Invest. Derm. 109: 613 only, 1997. 2. Copeland, N. G., Gilbert, D. J., Li, K., Sawamura, D., Giudice, G. J., Chu, M.-L., Jenkins, N. A., Uitto, J. Chromosomal localization of mouse bullous pemphigoid antigens, BPAG1 and BPAG2: identification of a new region of homology between mouse and human chromosomes. Genomics 15: 180-181, 1993. 3. Darling, T. N., Koh, B. B., Bale, S. J., Compton, J. G., Bauer, J. W., Hintner, H., Yancey, K. B. A deletion mutation in COL17A1 in five Austrian families with generalized atrophic benign epidermolysis bullosa represents propagation of an ancestral allele. J. Invest. Derm. 110: 170-173, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
142,344 Da
NCBI Official Full Name
collagen alpha-1(XVII) chain
NCBI Official Synonym Full Names
collagen type XVII alpha 1 chain
NCBI Official Symbol
COL17A1
NCBI Official Synonym Symbols
ERED; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
NCBI Protein Information
collagen alpha-1(XVII) chain
UniProt Protein Name
Collagen alpha-1(XVII) chain
UniProt Gene Name
COL17A1
UniProt Synonym Gene Names
BP180; BPAG2; LAD-1; 97 kDa LAD antigen; 97-LAD; LABD97
NCBI Description
This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.
Research Articles on COL17A1
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Product Notes
The COL17A1 col17a1 (Catalog #AAA1751443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Collagen XVII/COL17A1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen XVII/COL17A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 0.1-0.5 mug/ml IHC-P: 0.5-1 mug/ml Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Researchers should empirically determine the suitability of the COL17A1 col17a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XVII/COL17A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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