Western Blot (WB)
(Western blot analysis of COL17A1 Antibody (Center) in A375, A2058, HL-60, MCF-7 cell line lysates (35ug/lane). COL17A1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human COL17A1 Polyclonal Antibody | anti-COL17A1 antibody
COL17A1 Antibody (Center)
Western Blot (WB)
(Western blot analysis of COL17A1 Antibody (Center) in A375, A2058, HL-60, MCF-7 cell line lysates (35ug/lane). COL17A1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of COL17A1 Antibody (Center) in A375, A2058, HL-60, MCF-7 cell line lysates (35ug/lane). COL17A1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(COL17A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL17A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(COL17A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL17A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(COL17A1 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(COL17A1 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Ujiie,H., et.al., J. Immunol. 184 (4), 2166-2174 (2010)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]
Uniprot Description
COL17A1: May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. Defects in COL17A1 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Extracellular matrix
Chromosomal Location of Human Ortholog: 10q24.3
Cellular Component: collagen; hemidesmosome; integral to plasma membrane; endoplasmic reticulum lumen; plasma membrane; extracellular region; basement membrane; intercellular junction
Molecular Function: protein binding
Biological Process: collagen catabolic process; extracellular matrix disassembly; epidermis development; extracellular matrix organization and biogenesis; hemidesmosome assembly; cell-matrix adhesion
Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type
Research Articles on COL17A1
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Product Notes
The COL17A1 col17a1 (Catalog #AAA9204032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 475-504. The COL17A1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL17A1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the COL17A1 col17a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL17A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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