Rabbit anti-Human Collagen VIII alpha1 Polyclonal Antibody | anti-COL8A1 antibody

Collagen VIII alpha1 Antibody

Cat. Num. AAA9609219

• Gene Names: COL8A1; C3orf7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: Collagen VIII alpha1; Polyclonal Antibody; Collagen VIII alpha1 Antibody; C3orf7; CO8A1_HUMAN; COL8A1; Collagen alpha 1(VIII) chain [Precursor]; Endothelial collagen; MGC9568; Vastatin; anti-COL8A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Collagen VIII alpha1 antibody detects endogenous levels of total Collagen VIII alpha1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 744

• Applicable Applications for anti-COL8A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human Collagen VIII alpha1
• Subcellular Location: Secreted > Extracellular Space > Extracellular Matrix > Basement Membrane.
• Tissue Specificity: Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Collagen VIII alpha1 using LOVO whole cell lysates)

Related Product Information for anti-COL8A1 antibody

Function: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.
Subunit Structure: Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrahedron stabilized by central interacting C-terminal NC1 trimers.
Post-translational Modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.

NCBI and Uniprot Product Information

• NCBI GI #: 17738302
• NCBI GeneID: 1295
• NCBI Accession #: NP_001841.2
• NCBI GenBank Nucleotide #: NM_001850.4
• UniProt Accession #: P27658; Q53XI6; Q96D07; D3DN42
• Molecular Weight: Observed: 73 kDa; Predicted: 74 kDa
• NCBI Official Full Name: collagen alpha-1(VIII) chain
• NCBI Official Synonym Full Names: collagen type VIII alpha 1 chain
• NCBI Official Symbol: COL8A1
• NCBI Official Synonym Symbols: C3orf7
• NCBI Protein Information: collagen alpha-1(VIII) chain
• UniProt Protein Name: Collagen alpha-1(VIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL8A1
• UniProt Synonym Gene Names: C3orf7

NCBI Description

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

Uniprot Description

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.