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Western Blot (WB) (Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody)

Rabbit anti-Human COL5A3 Polyclonal Antibody | anti-COL5A3 antibody

COL5A3 Antibody

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
COL5A3; Polyclonal Antibody; COL5A3 Antibody; Collagen alpha-3(V) chain; anti-COL5A3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; In Phosphate Buffered Saline, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Applicable Applications for anti-COL5A3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:20000
Immunogen
Synthesized peptide derived from the N-terminal region of human COL5A3.
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of NIH-3T3 cells using COL5A3 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of NIH-3T3 cells using COL5A3 Polyclonal Antibody)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
172,121 Da
NCBI Official Full Name
collagen alpha-3(V) chain preproprotein
NCBI Official Synonym Full Names
collagen, type V, alpha 3
NCBI Official Symbol
COL5A3
NCBI Protein Information
collagen alpha-3(V) chain; collagen alpha-3(V) chain; pro-(alpha)3(V) collagen
UniProt Protein Name
Collagen alpha-3(V) chain
Protein Family
UniProt Gene Name
COL5A3
UniProt Entry Name
CO5A3_HUMAN

NCBI Description

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008]

Uniprot Description

COL5A3: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Belongs to the fibrillar collagen family.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: collagen type V; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; heparin binding; proteoglycan binding; extracellular matrix structural constituent

Biological Process: skin development; axon guidance; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; cell-matrix adhesion

Research Articles on COL5A3

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Product Notes

The COL5A3 col5a3 (Catalog #AAA7121898) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COL5A3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL5A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the COL5A3 col5a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL5A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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