Mouse NUP62 Monoclonal Antibody | anti-NUP62 antibody

NUP62 (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62) (HRP)

Cat. Num. AAA6153899

• Gene Names: NUP62; p62; IBSN; SNDI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUP62; Monoclonal Antibody; NUP62 (Nuclear Pore Glycoprotein p62; 62kD Nucleoporin; Nucleoporin Nup62) (HRP); anti-NUP62 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2D3
• Specificity: Recognizes human NUP62. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-NUP62 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa423-523 from NUP62 (NP_714941) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: LQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD*
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.11kD).)

Western Blot (WB)

(NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in PC-12.)

Western Blot (WB)

(NUP62 monoclonal antibody Western Blot analysis of NUP62 expression in Hela NE.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged NUP62 is ~1ng/ml as a capture antibody.)

Western Blot (WB)

(NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in NIH/3T3.)

Related Product Information for anti-NUP62 antibody

NUP62 is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This protein is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. The protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. All four variants represent identical protein.

Product Categories/Family for anti-NUP62 antibody

Antibodies; Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24497609
• NCBI GeneID: 23636
• NCBI Accession #: NP_714941
• NCBI GenBank Nucleotide #: NM_153719
• UniProt Accession #: P37198
• Molecular Weight: 57kDa
• NCBI Official Full Name: nuclear pore glycoprotein p62
• NCBI Official Synonym Full Names: nucleoporin 62
• NCBI Official Symbol: NUP62
• NCBI Official Synonym Symbols: p62; IBSN; SNDI
• NCBI Protein Information: nuclear pore glycoprotein p62
• UniProt Protein Name: Nuclear pore glycoprotein p62
• Protein Family: Nuclear pore glycoprotein
• UniProt Gene Name: NUP62
• UniProt Entry Name: NUP62_HUMAN

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

NUP62: Essential component of the nuclear pore complex. The N- terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI); also known as infantile bilateral striatal necrosis (IBSN) or familial striatal degeneration. SNDI is a neurological disorder characterized by symmetrical degeneration of the caudate nucleus, putamen and occasionally the globus pallidus, with little involvement of the rest of the brain. The clinical features include developmental regression, choreoathetosis, dystonia, spasticity, dysphagia, failure to thrive, nystagmus, optic atrophy and mental retardation. Belongs to the nucleoporin NSP1/NUP62 family.

Protein type: Nucleoporin; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: pore complex; spindle pole; nucleocytoplasmic shuttling complex; nuclear membrane; intracellular membrane-bound organelle; cytoplasm; nuclear envelope; nuclear pore; ribonucleoprotein complex

Molecular Function: ubiquitin binding; protein binding; receptor signaling complex scaffold activity; structural constituent of nuclear pore; PTB domain binding; chromatin binding; SH2 domain binding; thyroid hormone receptor binding; nucleocytoplasmic transporter activity

Biological Process: cell death; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; mRNA transport; viral reproduction; hormone-mediated signaling; positive regulation of transcription, DNA-dependent; mitotic nuclear envelope disassembly; regulation of signal transduction; nucleocytoplasmic transport; pathogenesis; viral infectious cycle; glucose transport; negative regulation of cell proliferation; protein transport; cell surface receptor linked signal transduction; positive regulation of epidermal growth factor receptor signaling pathway; negative regulation of programmed cell death; negative regulation of Ras protein signal transduction; viral transcription; transmembrane transport; regulation of Ras protein signal transduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; cell aging; hexose transport; carbohydrate metabolic process; gene expression; mitotic cell cycle; negative regulation of apoptosis

Disease: Striatonigral Degeneration, Infantile