Western Blot (WB)
(Anti-Collagen 5 alpha 3 Antibody at 1:1000 dilution + human heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 172 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human Collagen 5 alpha 3 Polyclonal Antibody | anti-COL5A3 antibody
Collagen 5 alpha 3 Antibody
Western Blot (WB)
(Anti-Collagen 5 alpha 3 Antibody at 1:1000 dilution + human heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 172 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Collagen 5 alpha 3 Antibody at 1:1000 dilution + human heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 172 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Grimwood J.,et al.Nature 428:529-535(2004).
Mann K.,et al.Biol. Chem. Hoppe-Seyler 373:69-75(1992).
Chen R.,et al.J. Proteome Res. 8:651-661(2009).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008]
Uniprot Description
COL5A3: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Belongs to the fibrillar collagen family.
Protein type: Secreted, signal peptide; Extracellular matrix; Secreted
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: collagen type V; endoplasmic reticulum lumen; extracellular region
Molecular Function: collagen binding; heparin binding; proteoglycan binding; extracellular matrix structural constituent
Biological Process: skin development; extracellular matrix disassembly; axon guidance; collagen catabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; cell-matrix adhesion
Research Articles on COL5A3
Similar Products
Product Notes
The COL5A3 col5a3 (Catalog #AAA9211407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 231-290. The Collagen 5 alpha 3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen 5 alpha 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB~~ 1:1000. Researchers should empirically determine the suitability of the COL5A3 col5a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen 5 alpha 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
