Rabbit anti-Human IL-4R/CD124 Polyclonal Antibody | anti-IL-4R/CD124 antibody

IL-4R/CD124 (Tyr497) polyclonal antibody

Cat. Num. AAA3000023

• Gene Names: IL4R; CD124; IL4RA; IL-4RA
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL-4R/CD124; Polyclonal Antibody; IL-4R/CD124 (Tyr497) polyclonal antibody; Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD_antigen: CD124; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP; IL4R; 582J2.1; anti-IL-4R/CD124 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: IL-4R/CD124(Tyr497) polyclonal antibody detects endogenous levels of HIST1H3A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Applicable Applications for anti-IL-4R/CD124 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000~1:2000
• Immunogen: Synthetic peptide, corresponding to Human IL-4R/CD124(Tyr497).
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of IL-4R/CD124(Tyr497) polyclonal antibody at 1:2000 dilutionLane1:HepG2 whole cell lysate(40ug)Lane2:MCF-7 whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug))

Related Product Information for anti-IL-4R/CD124 antibody

Background: The IL-2 receptor is a multicomponent complex consisting of three subunits, alpha,beta and gamma, each of which is required for high affinity binding of IL-2. The alpha chainfunctions primarily in binding IL-2, whereas the beta and gamma chains contribute toIL-2 binding and are essential to IL-2-induced activation of signaling pathwaysleading to T cell growth. Both IL-4R and IL-7R were initially described as single chain, high-affinity ligand-binding cytokine receptors. However, it is nowwell established that the IL-2Rgamma chain functions as a second subunit of thehigh affinity IL-4R and IL-7R receptors. Consequently, the originally describedsubunits of these latter receptors are now referred to as IL-4Ralpha and IL-7Ralpha,respectively, while the common subunit is referred to as gammac. Although the common gamma chain enhances ligand binding in these three cytokine receptors, ithas no capacity to bind these ligands on its own. There is evidence that thegammac chain is also a subunit of IL-13R.

NCBI and Uniprot Product Information

• NCBI GI #: 4557669
• NCBI GeneID: 3566
• NCBI Accession #: NP_000409.1
• NCBI GenBank Nucleotide #: NM_000418.3
• UniProt Accession #: P24394; Q96P01; Q9H181; Q9H182; Q9H183; Q9H184; Q9H185; Q9H186; Q9H187; Q9H188; B9EKU8; H3BSY5
• Molecular Weight: 89,658 Da
• NCBI Official Full Name: interleukin-4 receptor subunit alpha isoform a
• NCBI Official Synonym Full Names: interleukin 4 receptor
• NCBI Official Symbol: IL4R
• NCBI Official Synonym Symbols: CD124; IL4RA; IL-4RA
• NCBI Protein Information: interleukin-4 receptor subunit alpha; IL4R nirs variant 1; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain
• UniProt Protein Name: Interleukin-4 receptor subunit alpha
• UniProt Gene Name: IL4R
• UniProt Synonym Gene Names: IL4RA; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP
• UniProt Entry Name: IL4RA_HUMAN

NCBI Description

This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 16p12.1-p11.2

Cellular Component: extracellular space; integral to plasma membrane; receptor complex

Molecular Function: protein binding; interleukin-4 receptor activity; receptor signaling protein activity

Biological Process: ovulation; positive regulation of T-helper 2 cell differentiation; negative regulation of T-helper 1 cell differentiation; response to estrogen stimulus; production of molecular mediator of acute inflammatory response; immune response; defense response to protozoan; signal transduction; regulation of cell proliferation; positive regulation of macrophage activation

Disease: Ige Responsiveness, Atopic; Human Immunodeficiency Virus Type 1, Susceptibility To