Rabbit Cofilin Polyclonal Antibody | anti-CFL1 antibody

Anti-Cofilin Antibody

Cat. Num. AAA176848

• Gene Names: CFL1; CFL; HEL-S-15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Cofilin; Polyclonal Antibody; Anti-Cofilin Antibody; Cofilin-1; cofilin 1 (non-muscle); 18 kDa phosphoprotein antibody; CFL 1 antibody; CFL antibody; CFL1 antibody; COF1_HUMAN antibody; Cofilin 1 antibody; Cofilin 1 non muscle antibody; Cofilin antibody; Cofilin non muscle isoform antibody; Cofilin-1 antibody; non-muscle isoform antibody; p18 antibody; anti-CFL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 166

• Applicable Applications for anti-CFL1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Immunogen: E Coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Cofilin Picoband antibody, MBS176848-1.JPGIHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-Cofilin Picoband antibody, MBS176848-2.JPGIHC(P): Human Mammary Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-Cofilin Picoband antibody, MBS176848-3.JPGIHC(P): Mouse Lung Tissue )

Immunohistochemistry (IHC)

(Anti-Cofilin Picoband antibody, MBS176848-4.JPGIHC(P): Rat Lung Tissue )

Western Blot (WB)

(Anti-Cofilin Picoband antibody, MBS176848-5.jpgAll lanes: Anti-Cofilin(MBS176848) at 0.5ug/mlWB: Recombinant Human Cofilin Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD )

Related Product Information for anti-CFL1 antibody

Description: Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P, ELISA in Human, Mouse, Rat.
Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH, phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

NCBI and Uniprot Product Information

• NCBI GI #: 116848
• NCBI GeneID: 1072
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: Cofilin-1
• NCBI Official Synonym Full Names: cofilin 1 (non-muscle)
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL; HEL-S-15
• NCBI Protein Information: cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL; p18
• UniProt Entry Name: COF1_HUMAN

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; nuclear matrix; focal adhesion; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: axon guidance; actin filament depolymerization; platelet activation; positive regulation of actin filament depolymerization; response to virus; negative regulation of cell size; cytoskeleton organization and biogenesis; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; establishment of cell polarity; protein import into nucleus; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis