Chicken Total Immunoglobulin ELISA Kit | TIG elisa kit

Chicken Total Immunoglobulin ELISA Kit

Cat. Num. AAA743332

• Reactivity: Chicken
• Synonyms: Total Immunoglobulin; Chicken Total Immunoglobulin ELISA Kit; TIG elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 mg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIG elisa kit

Intended Uses: This TIG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken TIG. This ELISA kit for research use only!

Principle of the Assay: TIG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TIG antibody and an TIG-HRP conjugate. The assay sample and buffer are incubated together with TIG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIG concentration since TIG from samples and TIG-HRP conjugate compete for the anti-TIG antibody binding site. Since the number of sites is limited, as more sites are occupied by TIG from the sample, fewer sites are left to bind TIG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TIG elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 159030459
• UniProt Accession #: A8YMD6
• Molecular Weight: 51,856 Da
• NCBI Official Full Name: tig
• UniProt Protein Name: Trigger factor
• Protein Family: Tiggrin
• UniProt Gene Name: tig
• UniProt Synonym Gene Names: TF
• UniProt Entry Name: A8YMD6_MICAE

Uniprot Description

Function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase

By similarity. HAMAP MF_00303

Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0). HAMAP MF_00303

Subcellular location: Cytoplasm. Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm

By similarity. HAMAP MF_00303

Domain: Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own

By similarity. HAMAP MF_00303

Sequence similarities: Belongs to the FKBP-type PPIase family. Tig subfamily. HAMAP MF_00303Contains 1 PPIase FKBP-type domain. HAMAP MF_00303